Cloning and Analysis of NBS Type Resistance Gene Analogs in 'Chongtian Peach'(Prunus Persica L. Batch)

Yang Chunlei;Bu Lulu;Zhang Shijie;Li Chunyan;Luo Xinghui;Zhang Hongchang;Huang Guoyan;Liu Yan;Zhi Shi;Yang Zhengan;Yang Rongping;Yunnan Agricultural University;Gardening Station of Weishan County Agricultural Bureau;Agricultural Comprehensive Service Center of Tao Yuan Town,Yongsheng County;The Apple Industry Research Institute in Zhaotong;The Fruit and Vegetable Technology Extension Station in Mengzi;Plant Protection and Quarantine Station of Diqing Tibetan Autonomous Prefecture;

Chongtian peach;;NBS resistance gene;;Analogs;;Cloning and analysis

There are rich local peach resources in Yunnan,and'chongtian peach'is one of them.In order to find the germplasm resources with high resistance to disease in the local peach resources in Yunnan Province and provide a theoretical basis for the breeding of disease-resistant local peach varieties in Yunnan Province,in this study,the design of degenerate primers was based on the NBS-LRR class conservative sequence of known plant resistance genes.Taking'chongtian peach'as a test material,PCR amplification,cloning and sequencing of its genomic DNA were conducted.A total of 5 NBS class resistant gene analogs(RGAs)were obtained,and the size was about 500 bp.Sequence analysis showed that these 5 RGAs were highly homologous to some of the diseaseresistant proteins in the plants of peach subgenus.Some of the RGAs genes were highly homologous to the gene Prf against bacterial wilt of tomato and the N gene of tobacco mosaic virus.Therefore,it could be confirmed that there were abundent NBS-LRR class resistance genes in the plants of Prunus persica,which might lay the foundation for further breeding of disease-resistant local peach varieties in Yunnan Province.