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当前位置: 首页 > 文章 > 川中黑山羊RBP4基因cDNA克隆及序列分析 湖北农业科学 2014,53 (3) 211-214+236
Position: Home > Articles > Cloning and Sequence Analysis of Chuanzhong Black Goat RBP4 Gene Hubei Agricultural Sciences 2014,53 (3) 211-214+236

川中黑山羊RBP4基因cDNA克隆及序列分析

作  者：
龙石太;吴宪红;胡亮;字向东
单  位：
西南民族大学生命科学与技术学院
关键词：
川中黑山羊;RBP4基因;分子克隆;序列分析
摘  要：
以川中黑山羊肝脏组织总RNA为模板,通过RT-PCR技术对黑山羊RBP4基因进行克隆测序及序列分析。结果表明,所克隆的665 bp片段为预期的川中黑山羊RBP4基因cDNA序列,包含整个CDS区,该编码区长为606 bp,编码201个氨基酸。用DNAMAN软件进行同源性比对,结果显示,川中黑山羊RBP4基因编码区与GenBank中报道的牛、猪、马、黑猩猩、小鼠、大鼠、人的同源性分别是98.5%、91.3%、92.2%、91.4%、83.0%、82.3%、91.3%;氨基酸序列同源性分别为97.5%、91.5%、93.5%、91.5%、83.6%、83.6%、91.5%。利用NJ法和MP法以该基因编码区核苷酸序列构建物种间分子进化树,其结果基本一致。聚类结果与遗传距离大小一致,表明RBP4基因编码区适用于构建物种间分子系统进化树。
译  名：
Cloning and Sequence Analysis of Chuanzhong Black Goat RBP4 Gene
作  者：
LONG Shi-tai;WU Xian-hong;HU Liang;ZI Xiang-dong;College of Life Science and Technology,Southwest University of Nationalities;
关键词：
Chuanzhong black goat;;RBP4 gene;;molecular cloning;;sequence analysis
摘  要：
The total RNA was extracted from the liver of Chuanzhong black goat and the cDNA of RBP4 was obtained by the reverse transcription PCR(RT-PCR). The purified PCR product was cloned and the sequence was analyzed. The results showed that the 665 bp product was the cDNA of goat RBP4 gene, containing the complete CDS. The size of the goat RBP4 gene coding region was 606 bp, encoded 201 amino acids. Comparing RBP4 nucleotide sequences and deduced amino acid sequences of coding region of goat with those of other species including cow, swine, horse, chimpanzee, mouse, rat, human by DNAMAN software, the homologies of nucleotide sequences of the coding region of RBP4 gene were 98.5% 、91.3% 、 92.2% 、91.4% 、83.0% 、82.3% and 91.3%. The homologies of the deduced amino acid sequences of the coding region were 97.5%、91.5%、93.0%、91.5%、83.6%、83.6% and 91.5%, respectively. The molecular phylogenetic tree among species were constructed according to the nucleotide sequence of the coding region of RBP4 gene by the way of NJ and MP, the results between NJ and MP were basically consistent. This phylogenetic clustering was identical to that of the genetic distance and the zoological classification, indicating that the RBP4 gene was also suitable to construct molecular phylogenetic tree among different species.

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