中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 3株瘤胃厌氧真菌的分离与鉴定 黑龙江畜牧兽医 2015 (13) 167-168+171+290
Position: Home > Articles > Isolation and identification of three strains of rumen anaerobic fungi Heilongjiang Animal Science and Veterinary Medicine 2015 (13) 167-168+171+290

3株瘤胃厌氧真菌的分离与鉴定

作  者：
李敏;那仁图雅;周非帆;石彩霞;王海荣
单  位：
内蒙古农业大学动物科学学院
关键词：
瘤胃;厌氧真菌;分离;鉴定;纤维素降解率;亨氏滚管法
摘  要：
为了研究瘤胃厌氧真菌的纤维降解特性,试验采用体外亨氏滚管培养技术,从绵羊瘤胃液中分离得到3株(K、R、N)具有纤维降解能力的厌氧真菌,运用传统形态学并结合ITS序列同源性分析对其进行鉴定。结果表明:K为Neocallimastix属菌,R为Piromyces属菌,N为Cyllamyces属菌。同一菌株在36小时时降解率均极显著低于72小时(P<0.01),在36小时时菌株K、R、N三者间差异不显著(P>0.05),72小时时菌株K的降解率显著高于菌株R和菌株N(P<0.05)。
译  名：
Isolation and identification of three strains of rumen anaerobic fungi
作  者：
LI Min;NA REN Tu-ya;ZHOU Fei-fan;SHI Cai-xia;WANG Hai-rong;College of Animal Science,Inner Mongolia Agricultural University;
关键词：
rumen;;anaerobic fungi;;isolation;;identification;;cellulose degradation rate;;Hungate roll tube method
摘  要：
To study fibre degradation characteristics of rumen anaerobic fungi,three strains( K,R and N) of anaerobic fungi with the ability to degrade the fiber were isolated and obtained from rumen fluid of Inner Mongolia sheep using the Hungate roll tube method. The three strains were identified using traditional morphology combined with the homology analysis of internal transcribed space( ITS) sequence. The results showed that the three strains including K,R and N strains were identified as Neocallimastix spp,Piromyces spp,and Cyllamyces spp,respectively. The degradation rate at 36 h was highly significantly lower( P < 0. 01) than that at 72 h in the same strain. There were no significant differences( P > 0. 05) in the degradation rates at 36 h among the K,R and N strains,but the degradation rate of strain K was significantly higher( P <0. 05) than that of the R and N strains.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46