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Position: Home > Articles > Purification Process of Total Saponins in Abrus cantoniensis Using AB-8 Macroporous Resin Journal of Henan Agricultural Sciences 2013,42 (3) 150-153

AB-8大孔树脂纯化鸡骨草总皂苷的工艺研究

作  者:
廖春燕;黄瑶;杨晓
单  位:
广西科技大学生物与化学工程学院
关键词:
鸡骨草;总皂苷;AB-8大孔树脂;纯化
摘  要:
为了提高鸡骨草总皂苷的纯化效率,对AB-8大孔树脂纯化鸡骨草总皂苷的工艺条件及技术参数进行研究.以总皂苷吸附率、比吸附量和保留率为指标,考察AB-8大孔树脂对鸡骨草总皂苷的吸附及解吸性能.结果表明,鸡骨草皂苷提取液的上样质量浓度为1.00 g/L,pH值为6.0,上样速度为2.0 BV/h,上样量为2.0 BV;吸附平衡后用3.5 BV pH值为7.0的50%乙醇溶液洗脱,洗脱速度为4.0 BV/h,总皂苷保留率为82.8%,纯化后纯度达到75.18%.
译  名:
Purification Process of Total Saponins in Abrus cantoniensis Using AB-8 Macroporous Resin
摘  要:
In order to improve the purification efficiency of total saponins in Abrus cantoniensis,the conditions and parameters of purification process of total saponins using AB-8 macroporous adsorption resin were studied.The conditions of absorption and desorption were investigated with the indexes:absorption ratio and reserved rate of total saponins.The results showed that the sample loading solution was 1.00 g/L of 2.0 BV with pH value of 6.0 at the sample loading flow rate of 2.0 BV/h.The elution solvent was 3.5 BV of 50% ethanol solution with pH value of 7.0 at flow rate of 4.0 BV/h.The total saponins reserved rate was 82.8%,with the purity of 75.18%.

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