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当前位置: 首页 > 文章 > 紫外线诱变选育高产醋酸的醋酸菌研究 新疆农业科学 2012,49 (6) 1120-1126
Position: Home > Articles > Study on Breeding of Mutants of High-yield Acetic Acid bacteria by Ultraviolet Radiation Xinjiang Agricultural Sciences 2012,49 (6) 1120-1126

紫外线诱变选育高产醋酸的醋酸菌研究

作  者：
张蕾;郭龙;傅力
单  位：
新疆农业大学食品科学与药学学院;韩山师范学院生物系
关键词：
紫外线诱变;选育;醋酸菌;突变株
摘  要：
【目的】选育适合酿造杏渣醋的高产醋酸的醋酸菌株。【方法】对初始醋酸菌C1-0的生长曲线进行测定,对处于对数期的C1-0进行紫外线诱变,使诱变菌株的致死率达85%～90%,以产酸量为指标,选育高产醋酸的醋酸菌菌株,通过初筛和复筛,选育高产醋酸的醋酸菌菌株,并将其进行传代培养,测定产酸的稳定性。【结果】采用紫外线对培养至24 h的初始菌株C1-0照射20 s,使其致死率达到88.72%,获得了3株高产醋酸的醋酸菌突变株C1-11、C1-27、C1-30,产酸量分别达到37.29、38.80和36.60 g/L。这3株突变株传代5次,产酸量变化幅度较小。【结论】通过紫外线诱变的方法获得三株高产醋酸的醋酸菌株C1-11、C1-27、C1-30,比初始菌株C1-0产酸量分别提高4.5%、8.6%、2.6%,且遗传稳定性较好。
译  名：
Study on Breeding of Mutants of High-yield Acetic Acid bacteria by Ultraviolet Radiation
作  者：
ZHANG Lei1,GUO Long1,FU Li2(1.College of Food Science and Pharmaceutical Science,XinJiang Agricultural University,Urumqi 830052,China;2.Department of Biology,Hanshan Normal University,Chaozhou Guangzhou 521041,China)
关键词：
ultraviolet radiation;breeding;acetic acid bacteria;mutant
摘  要：
【Objective】The purpose of this study was to breed acetic acid bacteria with high-yield acetic acid for brewing vinegar from apricot dregs.【Method】The growth curve of acetic acid bacteria C1-0 was determined,then the strain of C1-0 was mutated by ultraviolet irradiation.And the lethal rate of mutants was set 85%-90%,The high-yield strains of acetic acid bacteria were screened.By means of the experiments of determining both pH value and acid content,the acid production for 5 generations of mutants was measured.【Result】 The strain of C1-0 was irradiated for 20s by UV when it came into logarithmic growth phase after it was incubated for 24 h,and,the lethal rate was 88.72%.Three high-yield mutants of C1-11,C1-27 and C1-30 have been obtained.The acid yield of C1-11,C1-27 and C1-30 were 37.29,38.80 and 36.60 g/L respectively.The acid yield for 5 generations of mutants changed little.【Conclution】The mutants of C1-11,C1-27,C1-30 with high-yield acetic acid by ultraviolet radiation were obtained,the acid yield of C1-11,C1-27,C1-30 were increased by 4.5%,8.6%,2.6% than C1-0,and genetic stability was good.

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