Exploration of Extracting Conditions and Composition Analysis on Purple Hybrid Rice Bran Oil

LUO Hai et al(Provincial Key Laboratory of Food Biotechnology of Sichuan,College of Bioengineering,Xihua University,Chengdu,Sichuan 610039)

Purple hybrid rice;Bran oil;Extraction;GC-MS;DPS

[Objective]This paper aimed to explore the conditions of extracting the bran oil in purple hybrid rice and to analyze its fatty acid composition.[Method]The purple hybrid rice bran oil was extracted by soxhlet extractor,then its fatty acid methyl esterized was characterized by GC-MS and clustered with those of peanut oil and colza oil by software DPS.[Result]The optimum extracting conditions were as follows:granularity of 50 meshes,8% water of bran,and material-liquid ratio of 1∶ 5.The bran oil extracted was decolorized with 4% hargil being added,90 ℃,and 30 min for the first time and followed 3% hargil being added,80 ℃,15 min for the second time by fast filtration under 0.08 MPa.Additionally,the oil was deodorized under 0.07 MPa and 170 ℃ for 1.5 min.Six saturated fatty acids with the content of 26.32% were mainly composed with hexadecanoic acid and stearic acid.And four dominant unsaturated fatty acids,a higher content of 67.66%,were oleic acid and linoleic acid.The dissimilarity about compositions and contents of purple hybrid rice was less than 0.94% when compared with those of II YOU 725 hybrid rice bran oil,while different from peanut oil and colza oil.[Conclusion] The quality of purple hybrid rice bran oil extracted by soxhlet extractor can attain to the standards of Tri-level rice bran oil in GB.Furthermore,it has better nutrition value and is worthy of being developed.