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Position: Home > Articles > Application of Microsatellite DNA on Analyzing the Homology of Related Species in Juglans L. Journal of Beijing University of Agriculture 2006,21 (3) 1-4

微卫星DNA在核桃属近缘种同源性分析上的应用

作  者：
郝艳宾;肖永强;齐建勋;齐实;程丽莉
单  位：
北京林业大学北京林业大学水土保持学院;北京市农林科学院林业果树研究所;北京林业大学生物科学与技术学院
关键词：
核桃属;微卫星标记;简单重复序列;多态信息含量
摘  要：
为了选择适合核桃遗传多样性研究的分子标记,运用woeste[1]等开发的30对黑核桃微卫星引物扩增核桃属(JuglansL.)26个样品,72.78%黑核桃微卫星引物能够在核桃属近缘种产生有效扩增。选取其中扩增条带清晰、有效性高、多态性高的17对引物分析核桃属12种。黑核桃微卫星在近缘种扩增位点等位基因数、期望杂合度和多态性信息含量比在黑核桃中有显著的下降趋势,但仍具有较高的多态水平,能够用于近缘种遗传多样性研究、品种指纹分析和核桃属种间关系研究。
译  名：
Application of Microsatellite DNA on Analyzing the Homology of Related Species in Juglans L.
作  者：
HAO Yan-bin~(1a,2),XIAO Yong-qiang~(1b*),QI Jian-xun~(2*),QI Shi~(1b),CHENG Li-li~(2)(1a.College of Biological Sciences and Technology,1b.College of soil and water conservation,Beijing Forestry University,Beijing 100083 china;2. Institute of forestry and Pomology,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100093,china)
关键词：
Juglans L.,microsatellite markers,SSR,polymorphism information content(PIC)
摘  要：
In order to find a suitable way for the polymorphism of J.regia L.,the genomic DNA variation of 26 accessions in related species of Juglans L.was analyzed by 30 pairs of primer which was isolated in Juglans nigra by Woeste~(\) et al.Among these primers,72.78% locus gave successful amplification in the related species which PCR-amplification present in the expected size range.17 SSR primers were selected based on the validity and polymorphism,and were analyzed for 12 related species.The numbers of alleles,the validity and PIC(polymorphism information content) had obviously declined than in J.nigra L..Although they have relatively high polymorphic levels for analyzling the genetic diversity in related species,fingerprinting and relationship in other Juglans species.

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