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Chinese Veterinary Science
2010
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马病毒性动脉炎和马鼻肺炎抗体时间分辨荧光免疫分析检测方法的建立
作 者：
郑小龙;梁成珠;朱来华;岳志芹;徐彪;王群;邓明俊;肖西志;姜帆;于业锋
单 位：
山东出入境检验检疫局
关键词：
时间分辨荧光免疫分析技术;马病毒性动脉炎;马鼻肺炎
摘 要：
以基因重组抗原包被96孔板,Eu3+标记兔抗马IgG,建立了马病毒性动脉炎和鼻肺炎抗体的时间分辨荧光免疫分析检测方法,对其特异性、灵敏性、稳定性进行了分析,并与进口ELISA试剂盒和微量血清中和试验进行了比较。结果显示,该方法交叉反应不明显,特异性好,灵敏度高于ELISA方法;该方法的批内和批间变异系数分别为2.68%～4.43%和4.21%～7.25%,Eu3+标记物可稳定保存5个月以上。证实,建立的马病毒性动脉炎和马鼻肺炎抗体的时间分辨荧光免疫分析检测技术灵敏度高,特异性和稳定性好,具有很好的应用前景。
译 名：
Development of time-resolved fluoroimmunoassay for detection of antibodies against equine viral arteritis and equine rhinopneumonitis
作 者：
ZHENG Xiao-long,LIANG Cheng-zhu,ZHU Lai-hua,YUE Zhi-qin,XU Biao,WANG Qun,DENG Ming-jun,XIAO Xi-zhi,JIANG Fan,YU Ye-feng(Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,Qingdao 266002,China)
关键词：
time-resolved fluoroimmunoassay;equine viral arteritis;equine rhinopneumonitis
摘 要：
Based on recombinant antigens and europium-labeled anti-horse IgG conjugate,time-resolved fluoroimmunoassay(TRFIA)methods were developed for detection of antibodies against equine arteritis virus and equine herpes viruses-1/4.Compared with indirect enzyme-linked immunosorbent assay and micro-serum neutralization test,the TRFIA methods exhibited equivalent specificity and high sensitivity,and maintained good reliability within its quantitative range,as evidenced by low coefficient of variation(2.68% to 7.25%).The developed methods were simple,sensitive and reliable,which had a good prospect of application.
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