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Position: Home > Articles > miR-24-3p promotes proliferation and inhibits apoptosis of porcine granulosa cells by targeting P27 Journal of Integrative Agriculture 2024,23 (4)

miR-24-3p promotes proliferation and inhibits apoptosis of porcine granulosa cells by targeting P27

作  者：
Shi, Shengjie;Zhang, Lutong;Wang, Liguang;Yuan, Huan;Sun, Haowei;Madaniyati, Mielie;Cai, Chuanjiang;Pang, Weijun;Gao, Lei;Chu, Guiyan
单  位：
Key Lab Anim Genet Breeding & Reprod Shaanxi Prov, Yangling 712100, Peoples R China;Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China
关键词：
miR-24-3p;granulosa cells;proliferation;apoptosis
摘  要：
Ovarian follicle development is associated with the physiological functions of granulosa cells (GCs), including proliferation and apoptosis. The level of miR-24-3p in ovarian tissue of high -yielding YorkshirexLandrace sows was significantly higher than that of low -yielding sows. However, the functions of miR-24-3p on GCs are unclear. In this study, using flow cytometry, 5-ethynyl-2-de-oxyuridine (EdU) staining, and cell count, we showed that miR-24-3p promoted the proliferation of GCs increasing the proportion of cells in the S phase and upregulating the expression of cell cycle genes, moreover, miR-24-3p inhibited GC apoptosis. Mechanistically, on-line prediction, bioinformatics analysis, a luciferase reporter assay, RT-qPCR, and Western blot results showed that the target gene of miR-24-3p in proliferation and apoptosis is cyclin-dependent kinase inhibitor 1B ( P27/CDKN1B ). Furthermore, the effect of miR-24-3p on GC proliferation and apoptosis was attenuated by P27 overexpression. These findings suggest that miR-24-3p regulates the physiological functions of GCs.

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