Position: Home > Articles > Effects of Recombinant UBC13 on mRNA Expressions of Inflammatory Cytokines in RAW264.7 Cells
Progress in Veterinary Medicine
2017
(6)
20-24
重组UBC13对RAW264.7炎症因子mRNA表达的影响
作 者：
张亮;扆妍妍;陶佳丽;段马魁;姜俊兵
单 位：
山西农业大学动物科技学院
关键词：
UBC13;小鼠巨噬细胞;炎症因子;基因表达
摘 要：
为了研究重组蛋白UBC13对RAW264.7小鼠巨噬细胞炎症因子mRNA表达量的影响,采用噻唑蓝(MTT)法检测UBC13对RAW264.7细胞活力的影响,qPCR法检测不同浓度UBC13(20、10、5μg/mL)在不同时间点(3、6、9、12、24h)对RAW264.7细胞产生的IL-6、IL-1β、TNF-α和COX-2 4种炎症因子mRNA表达量的影响。结果表明,在整个试验时间范围内IL-6、IL-1β、TNF-α和COX-2mRNA表达量呈现逐步降低的趋势;与细胞对照组组相比,在24h时IL-6、IL-1β、TNF-α和COX-2mRNA表达量显著降低(P<0.05)。说明重组蛋白UBC13作用于RAW264.7细胞24h时能够明显抑制RAW264.7细胞中IL-6、IL-1β、TNF-α和COX-2的mRNA表达,并且具有药物剂量依赖性。
译 名：
Effects of Recombinant UBC13 on mRNA Expressions of Inflammatory Cytokines in RAW264.7 Cells
作 者：
ZHANG Liang;YI Yan-yan;TAO Jia-li;DUAN Ma-kui;JIANG Jun-bing;College of Animal Science and Veterinary Medicine,Shanxi Agricultural University;
关键词：
UBC13;;RAW264.7;;inflammatory cytokine;;gene expression
摘 要：
To investigate the effects of recombinant UBC13 on mRNA expressions of the inflammatory cytokines in RAW264.7cells,the viability of cells was measured by using MTT assay.qPCR method was used to detect the effects of different concentrations(20,10,5μg/mL)of UBC13 on the mRNA expression levels of IL-6,IL-1β,TNF-αand COX-2at 3,6,9,12,24 h.The results showed that the mRNA expressions of IL-6,IL-1β,TNF-αand COX-2decreased gradually at the whole stage of the experiment.The mRNA expressions of IL-6,IL-1β,TNF-αand COX-2were significantly decreased in UBC13 treatment group at 24h(P<0.05),comparing with the cell group.Our results indicated that the UBC13 could significantly inhibits the mRNA expressions of IL-6,IL-1β,TNF-αand COX-2in RAW264.7cells at 24 hin a dose-dependent manner.
相似文章
-
UBC13对支气管哮喘模型小鼠Th2、Th17型细胞极化的影响 [郭音, 孟维金, 孙健康, 姜俊兵] 中国畜牧兽医 2020 (4) 1148-1155
