Position: Home > Articles > Antioxidant and Cholesterol-reducing Properties of Enterococcus gallinarum m661
FOOD SCIENCE
2013,34
(7)
157-161
鹑鸡肠球菌m661的抗氧化、降胆固醇作用
作 者：
刘长建;刘秋;姜波;闫建芳;孙天竹
单 位：
大连民族学院生命科学学院
关键词：
猪胃液;肠球菌属;16S rDNA;系统发育树;抗氧化作用
摘 要：
经厌氧培养,从猪胃液中分离得到1株生长良好的肠球菌m661,菌株能产乳酸、革兰氏染色阳性、接触酶阴性.经系统发育分析,结合菌落形态、细胞形态、生化反应实验,确定菌株m661为鹑鸡肠球菌.鹑鸡肠球菌m661具有抗氧化活性,对羟自由基(.OH)、DPPH自由基和超氧阴离子自由基(O-2.)的清除率与细胞浓度呈正相关.在细胞浓度为5*108CFU/mL时,菌株m661对.OH、DPPH自由基和O-2.的清除率分别为1.58%、36.6%和57.1%.菌株m661还具有清除培养基中34.4%的胆固醇、产生89.9mg/L苯乳酸等益生特性.菌株m661可作为益生菌进行开发利用.
译 名：
Antioxidant and Cholesterol-reducing Properties of Enterococcus gallinarum m661
单 位：
College of Life Science,Dalian Nationalities University,Dalian 116600,China
关键词：
porcine gastric juice%Enterococcus%16S rDNA%phylogenetic tree%antioxidant effect
摘 要：
An enterococcal strain named as m661 was isolated from porcine gastric juice.Strain m661 was found to be capable of producing lactic acid,Gram-positive and catalase-negative.Based on phylogenetic analysis,cellular and colonial morphology and biochemical test,m661 was identified as Enterococcus gallinarum.This strain had antioxidant potential,and its scavenging activity against hydroxyl,DPPH and superoxide anion radicals was in a cell density-dependent manner.At a cell density of 5 * 108 CFU/mL,the scavenging rates of stain m661 against hydroxyl,DPPH,and superoxide anion radicals were 1.58%,36.6% and 57.1%,respectively.In addition,m661 was found to reduce cholesterol by 34.4% in the culture medium and produce 89.9 mg/L phenyllactic acid,suggesting excellent health-benefiting effects.The results of this study demonstrate that strain m661 has great potential to be developed as a new probiotic resource.
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