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Position: Home > Articles > Revision of the quality standards of traditional Chinese medicine oral solution against post-weaning swine diarrhea Journal of Northeast Agricultural University 2008,39 (5) 96-99

抗仔猪腹泻中药口服液的质量标准研究

作  者:
孟倩;李继昌;潘晓影;丁良君
单  位:
东北农业大学动物医学学院
关键词:
仔猪腹泻;中药口服液;质量标准;薄层色谱;高效液相色谱
摘  要:
采用薄层色谱法对抗仔猪腹泻口服液中的黄连、黄芩、甘草药材进行定性鉴别,并应用高效液相色谱法对其中成分盐酸小蘖碱进行含量测定。结果显示,薄层色谱法均能对黄连、黄芩、甘草进行定性分析;盐酸小檗碱进样浓度在0.0032~0.016mg.mL-1范围内线性关系良好,平均加样回收率为99.2%(n=5),RSD为0.9%。结果表明,本试验所建立的方法准确、重现性好,可作为抗仔猪腹泻中药口服液的质量控制方法。
译  名:
Revision of the quality standards of traditional Chinese medicine oral solution against post-weaning swine diarrhea
作  者:
MENG Qian,LI Jichang,PAN Xiaoying,DING Liangjun (College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China)
关键词:
post-weaning swine diarrhea; traditional Chinese medicine oral solution; quality standards; TLC; HPLC
摘  要:
Rhizoma coptidis, Radix scutellariae, Radix glycytthizae in oral solution against post-weaning swine diarrhea were identified by TLC; the main content of berberine hydrochloride in the oral solution was assayed by HPLC. The results showed that Rhizoma coptidis, Radix scutellariae, Radix glycytthizae could be iden-tified by TLC; berberine hydrochloride showed a good linear relationship at the range of 0.0032~0.016 mg·mL-1(r=0.9996); the average recovery was 99.2%(n=5) and the RSD was 0.9%. The methed developed is accurate and has good reproducibility which could be used for the quality control of this oral solution.

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