Position: Home > Articles > The preparation and preliminary application of Saporin-Coupled dtSCT IGRP_(206-214) H-2K~d in NOD mice
Journal of Yangzhou University(Agricultural and Life Science Edition)
2016,37
(2)
12-16
IGRP_(206-214)dtSCT四聚体偶联皂草素的制备及初步应用
作 者：
王宇航;窦延;徐娅雯;马旻轩;李凯;潘兴元;钱莉;龚卫娟;季明春
单 位：
扬州大学医学院
关键词：
H-2Kd四聚体;CD8+T细胞;1型糖尿病;NOD鼠
摘 要：
以InsB15-23dtSCT原核表达载体为基础,制备dtSCT型IGRP206-214H-2Kd原核表达载体;将获取的融合蛋白IGRP206-214H-2Kd单体,生物素化制成IGRP206-214dtSCT四聚体,进一步将皂草素与IGRP206-214dtSCT四聚体偶联;流式检测皂草素偶联IGRP206-214dtSCT四聚体与NOD鼠脾脏淋巴细胞共孵育体系中IGRP206-214特异性细胞毒性T细胞(CTL)。结果表明:皂草素偶联IGRP206-214dtSCT四聚体与NOD鼠脾脏淋巴细胞共孵育后,IGRP206-214特异性CTL的频率与PBS对照组相比明显下降(P<0.001)。说明偶联皂草素的IGRP206-214dtSCT四聚体在体外能够靶向清除NOD鼠抗原特异性CTL。
译 名：
The preparation and preliminary application of Saporin-Coupled dtSCT IGRP_(206-214) H-2K~d in NOD mice
作 者：
WANG Yuhang;DOU Yan;XU Yawen;MA Minxuan;LI Kai;PAN Xingyuan;QIAN Li;GONG Weijuan;JI Mingchun;Coll of Med,Yangzhou Univ;
关键词：
H-2Kd tetramer;;CD8+T cells;;type 1 diabetes mellitus;;NOD mice
摘 要：
On the basis of InsB15-23 dtSCT prokaryotic expression vector,dtSCT type of IGRP206-214H-2Kd prokaryotic expression vector was construct.The obtained fusion protein IGRP206-214 H-2Kd monomer was biotinylated,then the IGRP206-214 dtSCT tetramer was coupled with saporin.The spleen lymphocytes from NOD were co-cultured with saporinCoupled dtSCT IGRP206-214H-2Kd tetramer and the IGRP206-214-specific CTLs were detected by Flow Cytometry.Co-incubated NOD spleen lymphocytes together with Saporin Coupled dtSCT IGRP206-214H-2Kdtetramer,the IGRP206-214-specific CTLs significantly decreased compared to the PBS control group(P<0.001,n=6).These results showed that Saporin Coupled dtSCT IGRP206-214H-2Kd tetramer can selectively clear NOD specific CTLs in vitro.
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