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当前位置: 首页 > 文章 > 反复冻融增强葛仙米藻胆蛋白抗氧化特性研究 食品科学 2011,32 (23) 121-124
Position: Home > Articles > Antioxidant Enhancement of Phycobiliprotein from Nostoc sphaeroides Ku ting by Repeated Freeze-Thawing Treatment FOOD SCIENCE 2011,32 (23) 121-124

反复冻融增强葛仙米藻胆蛋白抗氧化特性研究

作  者：
程超;薛峰;汪兴平;潘思轶
单  位：
湖北民族学院生物科学与技术学院;石河子质量与计量检测所;华中农业大学食品科技学院
关键词：
反复冻融;ABTS+.;红细胞溶血抑制;脂质过氧化抑制
摘  要：
研究反复冻融处理对葛仙米藻胆蛋白抗氧化特性的影响,测定其对抗氧化活性和对H2O2诱导的红细胞溶血和脂质过氧化的抑制作用。结果发现:经过反复冻融处理的葛仙米藻胆蛋白以pH7.3的50mmol/L的磷酸氢二钾-氢氧化钠为溶剂溶解的样品对ABTS+.的抗氧化活性和对H2O2诱导的红细胞溶血和脂质过氧化抑制作用强于其他处理,同时对铁离子的还原能力也较强,主要原因是反复冻融处理增加了葛仙米藻胆蛋白的溶出量,改变其中藻蓝蛋白和藻红蛋白的比例关系。
译  名：
Antioxidant Enhancement of Phycobiliprotein from Nostoc sphaeroides Ku ting by Repeated Freeze-Thawing Treatment
作  者：
CHENG Chao1,2,XUE Feng1,WANG Xing-ping2,PAN Si-yi1,(1.College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China; 2.Biological Scientific and Technical College,Hubei University for Nationalities,Enshi 445000,China)
关键词：
repeated freeze-thawing treatmen;ABTS+·;red blood cell homelysis inhibition;lipid peroxidation inhibition
摘  要：
The present study aimed to investigate the effect of repeated freeze-thawing treatment on the antioxidant capacity of phycobiliprotein from Nostoc sphaeroides Ku ting.Phycobiliproteins extracted from Nostoc sphaeroides Ku ting with and without repeated freeze-thawing treatment were dissolved separately in double distilled water and pH 7.3,50 mol/L dipotassium phosphate-sodium hydroxide buffer,resulting in four different samples: RFTPW,RFTPP,NRFTPW and NRFTPP,respectively.The antioxidant activity of the four samples was comparatively assessed by ABTS+ radical scavenging assay,FRAP assay and inhibition assay against erythrocyte hemolysis and lipid peroxidation both induced by H2O2.RFTPP was found to have greater inhibitory effects against ABTS+ radicals,erythrocyte hemolysis and lipid peroxidation than other three samples.Moreover,its ferric reducing activity was stronger.These results may be due to an increase in the dissolved phycobiliprotein caused by repeated freeze-thawing treatment,altering the ratio between phycocyanin and phycoerythrin.

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