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当前位置: 首页 > 文章 > 明党参和川明参种间遗传分化和系统关系的分子标记和ITS序列分析 浙江大学学报(农业与生命科学版) 2008,34 (5) 473-481
Position: Home > Articles > Analysis of genetic differentiation and phylogenetic relationship between Changium smyrnioides and Chuanminshen violaceum using molecular markers and ITS sequences. Journal of Zhejiang University (Agriculture and Life Sciences) 2008,34 (5) 473-481

明党参和川明参种间遗传分化和系统关系的分子标记和ITS序列分析

作  者：
陶晓瑜;桂先群;傅承新;邱英雄
单  位：
安徽省天堂寨国家森林公园;浙江大学生命科学学院植物系统进化与生物多样性实验室
关键词：
明党参;川明参;分子标记;ITS序列;系统关系
摘  要：
为了阐明明党参(Changiumsmyrnioides)和川明参(Chuanminshen violaceum)种间遗传分化和系统关系,利用RAPD、ISSR和ITS序列分析方法对明党参和川明参不同居群,以及芹亚科(Apioideae)6个族的一些代表种进行研究.RAPD和ISSR分析表明,明党参与川明参种间在全基因组水平出现明显的遗传分化.ITS序列分析则表明,明党参的6个居群在ITS序列上稍有差异,ITS-1的GC含量为58.3%~59.2%,ITS-2的GC含量为54.3%~56.6%.ITS-1的长度范围为215~219 bp,ITS-2的长度范围为224~227 bp.川明参与明党参种间的ITS序列也出现明显分化.芹亚科6个族一些代表种的ITS序列系统发育分析结果支持明党参置于美味芹族(Smyrnieae Koch)的分类地位.在ITS系统树上,川明参与明党参为姐妹类群的置信度为100%,因此,提出将川明参置于美味芹族的分类学处理.
译  名：
Analysis of genetic differentiation and phylogenetic relationship between Changium smyrnioides and Chuanminshen violaceum using molecular markers and ITS sequences.
作  者：
TAO Xiao-yu1,GUI Xian-qun2,FU Cheng-xin1,QIU Ying-xiong1(1.Lab of Plant Systematic Evolution and Biodiversity,College of Life Sciences,Zhejiang University,Hangzhou 310058,China;2.Tiantangzhai National Forest Park,Lu'an,Anhui 237343,China)
关键词：
Changium smyrnioides;Chuanminshen violaceum;molecular marker;ITS(internal transcribed spacer) sequence;phylogenetic relationship
摘  要：
The data from RAPD,ISSR analysis,and the sequences of the nrDNA internal transcribed spacer(ITS) were used to evaluate the genetic differentiation and phylogenetic relationship between Changium smyrnioides and Chuanminshen violaceum.Six wild populations of C.smyrnioides and one cultivated population of C.violaceum were studied by ISSR and RAPD analysis.The results show that the genetic variation of C.smyrnioides was high and the majority of genetic variation occurred among populations.Cluster analysis(UPGMA) indicated that substantially genetic divergence was found between C.smyrnioides and C.violaceum at DNA level.The sequences of ITS-1 in six populations of C.smyrnioides ranged from 215 bp to 219 bp in size and those of ITS-2 from 224 bp to 227 bp.The G+C contents of ITS-1 varied from 58.3% to 59.2%,and those of ITS-2 varied from 54.3% to 56.6%.ITS sequence analysis also revealed certain differences between C.smyrnioides and C.violaceum.ITS regions from C.smyrnioides,C.violaceum and relative species in Apioideae were sequenced and analyzed.Results strongly support the systematic position of C.smyrnioides.In the ITS phylogenetic trees,C.violaceum was sister to C.smyrnioides with high bootstrap values(100%),suggesting that C.violaceum should be placed in Smyrnieae Koch.

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