Position: Home > Articles > The Determinnation of Steroidal Alkaloid Content in Solanum nigrum L
FOOD SCIENCE
2006,27
(8)
224-227
龙葵中甾体类生物总碱的含量测定
作 者：
蒋新宇;杨辉;赵宇
单 位：
中南大学化学化工学院;昌宏医药研究所有限公司
关键词：
澳洲茄胺;龙葵生物碱;高效液相色谱;中草药提取
摘 要：
目的:建立龙葵甾体类生物总碱的提取和检测方法。方法:采用醇提-酸醇水解法提取龙葵内的澳洲茄胺,定性定量分析澳洲茄胺;以澳洲茄胺为参照品,表征龙葵中甾体类生物总碱的含量。结果:建立了龙葵甾体类生物总碱的提取和检测方法。结论:新建立的甾体类生物总碱提取和检测方法可快速、准确地测定龙葵中甾体类生物总碱的含量;以澳洲茄胺为苷元的甾体类生物总碱主要富集在龙葵未成熟果实中,龙葵茎叶内含量小。
译 名：
The Determinnation of Steroidal Alkaloid Content in Solanum nigrum L
作 者：
JIANG Xin-yu1,YANG Hui1,*,ZHAO Yu2(1.College of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China;2.Changhong Medicine Research Company,Zhuhai 519085,China)
关键词：
solancarpidine;solalum nigrum L alkaloid;HPLC;herb extraction
摘 要：
Objective:To establishing a method of steroidal alkaloids extraction and detecting also assay method in Solanum nigrum L.Method:After extracting solancarpidine by the method of ethanol extracting-hydrolyzing with acid and ethanol,solan carpidine is analyzed;By detecting solancarpidine,the content of steroidal alkaloids is also to be learned by using solancarpidine as reference.Conclusion:The content of steroidal alkaloids is determined rapidly and accurately by the new method of steroidal alkaloids's extraction and detecting;the steroidal alkaloids whose hydrolysate is solancarpidine in Solalum nigrum L has been found mainly in its immature fruits,but a little in stems and leaves.
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