当前位置: 首页 > 文章 > 抗苄嘧磺隆雨久花乙酰乳酸合成酶突变的研究 植物保护 2015 (5) 88-93
Position: Home > Articles > Mutations in acetolactate synthase confer bensulfuron-methyl herbicide resistance in Monochoria korsakowii Plant Protection 2015 (5) 88-93

抗苄嘧磺隆雨久花乙酰乳酸合成酶突变的研究

作  者:
张云月;卢宗志;李洪鑫;崔海兰
单  位:
吉林省农业科学院植物保护研究所;中国农业科学院植物保护研究所
关键词:
苄嘧磺隆;乙酰乳酸合成酶;抗药性;雨久花
摘  要:
本研究采用cDNA末端快速扩增技术(RACE)结合RT-PCR方法克隆抗苄嘧磺隆雨久花生物型和敏感性雨久花生物型乙酰乳酸合成酶(ALS)基因cDNA序列,并对测序结果进行比对分析。结果表明:与敏感性的雨久花ALS相比,公主岭(GZL)抗性生物型中第197位脯氨酸突变为组氨酸,第556位亮氨酸突变为苯丙氨酸;柳河(LH)抗性生物型中第358位天冬酰胺突变为天冬氨酸;磐石市(PS)抗性生物型中第525位缬氨酸突变为异亮氨酸。分析表明,高度保守区Domain A的第197位氨基酸残基的突变可能是导致公主岭稻区雨久花产生抗药性的主要原因之一,而其他抗性生物型抗性产生的原因有待进一步研究。
译  名:
Mutations in acetolactate synthase confer bensulfuron-methyl herbicide resistance in Monochoria korsakowii
作  者:
Zhang Yunyue;Lu Zongzhi;Li Hongxin;Cui Hailan;Institute of Plant Protection,Jilin Academy of Agricultural Sciences,Key Laboratory of Integrated Pest Management on Crops in Northeast,Ministry of Agriculture;Institute of Plant Protection,Chinese Academy of Agricultural Sciences;
关键词:
bensulfuron-methyl;;acetolactate synthetase;;herbicide resistance;;Monochoria korsakowii
摘  要:
Acetolactate synthetase(ALS)gene sequences were cloned from Monochoria korsakowii resistant(R)and susceptible(S)biotypes against bensulfuron-methyl using the rapid amplification of cDNA ends(RACE)and RT-PCR.Sequencing data revealed that Pro-197-His mutation and Leu-556-Phe mutation occurred in population GZL,while Asn-358-Asp mutation occurred in population LH,and Val-525-Ile mutation in population PS.The Pro-197-His mutation may be responsible for the resistance to bensulfuron-methyl in population GZL of M.korsakowii.The functions of the other three mutations in the R biotype need to be further investigated.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊