关键词：
苜蓿素;抗炎;奶牛乳腺上皮细胞;脂多糖;细胞增殖
摘 要：
本试验旨在研究苜蓿素对脂多糖(LPS)刺激下体外培养奶牛乳腺上皮细胞活性和炎症相关基因表达的影响。将体外培养的奶牛乳腺上皮细胞分成6组,对照组采用基础培养基,试验组在基础培养基中分别加入1μg/m L LPS(L组)、1μg/m L LPS和2.5μg/m L苜蓿素(L+2.5组)、1μg/m L LPS和5.0μg/m L苜蓿素(L+5组)、1μg/m L LPS和10.0μg/m L苜蓿素(L+10组)以及1μg/m L LPS和15.0μg/m L苜蓿素(L+15组),培养24 h。结果表明:1)与对照组相比,L组奶牛乳腺上皮细胞相对增殖率极显著降低(P<0.01),而L+2.5组和L+10组则极显著升高(P<0.01);2)与L组相比,L+10组和L+15组细胞超氧化物歧化酶活性显著升高(P<0.05),而乳酸脱氢酶活性和一氧化氮浓度显著降低(P<0.05);3)与L组相比,L+5组和L+10组细胞的白细胞介素8、白细胞介素1β、肿瘤坏死因子α、Toll样受体2和Toll样受体4的相对表达量均极显著降低(P<0.01),L+5组细胞的髓样分化因子88相对表达量均显著降低(P<0.05)。结果提示,在LPS刺激下,添加苜蓿素能够提高奶牛乳腺上皮细胞活性和抗氧化性能,抑制炎症相关基因的表达,发挥抗炎作用。
译 名：
Effects of Tricin on Viability and Expressions of Genes Related to Inflammation of Bovine Mammary Epithelial Cells Stimulated by Lipopolysaccharide in Vitro
作 者：
ZHAN Jinshun;GU Deping;HU Lizhen;ZHONG Xiaojun;HUO Junhong;Institute of Animal Husbandry and Veterinary,Jiangxi Academy of Agricultural Sciences;
关键词：
tricin;;anti-inflammatory;;bovine mammary epithelial cell;;lipopolysaccharide;;cell proliferation
摘 要：
The aim of this experiment was to investigate the effects of tricin on viability and expressions of genes related to inflammation of bovine mammary epithelial cells( BMECs) stimulated by lipopolysaccharide( LPS) in vitro. The bovine mammary epithelial cells cultured in vitro were divided into 6 groups and cultured for 24 h. The BMECs cultured in basal culture medium were used as the control group. The BMECs cultured in basal culture medium supplemented with 1 μg/mL LPS,1 μg/mL LPS +2.5 μg/mL tricin,1 μg/mL LPS +5.0 μg/mL tricin,1 μg/mL LPS+10.0 μg/mL tricin and 1 μg/mL LPS+15.0 μg/mL tricin were used as L,L+2.5,L+5,L+10 and L+15 groups,respectively. The results showed as follow : 1) compared with the control group,the relative growth rate of BMECs in L group was significantly decreased( P<0.01),whereas that in L+2.5 and L+10 groups was significantly increased( P<0.01); 2) compared with L group,the activity of superoxide dismutase of cells was significantly increased( P<0.05),but the activity of lactate dehydrogenase and the concentrations of nitric oxide and malondialdehyde of cells were significantly reduced in L+10 and L+15 groups( P<0.05); 3) compared with L group,the relative expression levels of interleukin-8,interleukin-1β,tumor necrosis factor-α,Toll-like receptor 2 and Toll-like receptor 4 in L+5 and L+10 groups were significantly decreased( P<0.01),and the relative expression level of myeloid differentiation primary response 88 in L+5 group was significantly decreased( P<0.05). The results indicated that the supplementation of tricin can enhance the viability and oxidation resistance of BMECs stimulated by LPS,and plays a role of anti-inflammatory effect by inhibiting gene expressions related to inflammation.
