当前位置: 首页 > 文章 > 蛋鸡禽白血病的实验室诊断 贵州畜牧兽医 2018,42 (6) 26-28
Position: Home > Articles > Laboratory Diagnosis of Avian Leukemia Guizhou Journal of Animal Husbandry & Veterinary Medicine 2018,42 (6) 26-28

蛋鸡禽白血病的实验室诊断

作  者:
晏晓洪;胡安东;贺欣薇;温贵兰;文明;皮泉;张霞
单  位:
贵州省动物疫病预防控制中心;贵州大学动物科学学院
关键词:
禽白血病;细菌分离培养;病毒PCR检测
摘  要:
对开阳县某蛋鸡场发生的疫病通过发病情况调查、剖检病变观察、细菌分离培养和相关病毒PCR检测。结果:疫病主要发生于160日龄以上鸡群,病变以肝脏和脾脏显著肿大为特征;细菌分离培养未见菌落生长; PCR检测可见禽白血病病毒特异性DNA片段,但未检出鸡马立克氏病病毒特异性DNA片段。综合诊断为禽白血病病毒感染。
译  名:
Laboratory Diagnosis of Avian Leukemia
作  者:
Yan Xiaohong;Hu ANDong;He Xinwei;Wen Guilan;Wen Ming;Pi Quan;Zhang Xia;College of Animal Science,Guizhou University;Guizhou Centre for Prevention and Control of Animal Disease;
关键词:
Avian Leukosis;;Isolation and Culture of Bacteria;;PCR Detection of Virus
摘  要:
The dead chicken cases,occurred in a laying hen farm in Kaiyang county,were diagnosed by incidence investigation,observation of diseased lesion,isolation and culture of bacteria and PCR detection of virus nucleic acid.Results:this cases mainly occurred in chickens over 160 days old,and the dissection lesion were characterized by marked enlargement of liver and spleen in the sick chickens.No bacterial growth were observed after isolation and culture.The specific DNA fragments of ALV were detected by RT-PCR in liver of the sick chickens,but the fragments of MDV were not detected.These results suggested that this case was caused by avian leukosis virus infection.

相似文章

计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊