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Position: Home > Articles > A Study of Tissue Culture of Cinnamomum longepaniculatum Journal of Sichuan Forestry Science and Technology 2019 (2) 42-47

油樟组织培养技术研究

作  者:
李佳蔓;黄振;杨汉波;陈炙;邢文曦;靳伟;郭洪英
单  位:
四川省林业科学研究院
关键词:
油樟;组织培养;培养基;外源激素
摘  要:
以当年生油樟枝条为研究对象,探索了消毒剂种类、浓度及其作用时间、MS培养基、6-BA、NAA、IBA等5种因素对油樟茎段组织培养的影响,分析筛选出了一套能够产出具有优良素质油樟种苗的组织培养技术。结果表明:油樟茎段经10%84处理15min或15%84处理10 min两种方法进行消毒均能获得污染率<15%,且成活率>70%的无菌外植体;适合不定芽诱导的培养基为:蔗糖30 g·L~(-1)+卡拉胶6 g·L~(-1)+MS+6-BA 1.0 mg·L~(-1)+IBA 0.1 mg·L~(-1);适合不定芽增殖继代的培养基:蔗糖30 g·L~(-1)+卡拉胶6 g·L~(-1)+MS+6-BA 1.0 mg·L~(-1)+NAA 0.10 mg·L~(-1)+IBA 0.2 mg·L~(-1);生根培养基:蔗糖15 g·L~(-1)+卡拉胶6 g·L~(-1)+1/2MS+IBA 1.5 mg·L~(-1)+NAA 1.0 mg·L~(-1)。
译  名:
A Study of Tissue Culture of Cinnamomum longepaniculatum
作  者:
LI Jia-man;HUANG Zhen;YANG Han-bo;CHEN Zhi;XING Wen-xi;JIN Wei;GUO Hong-ying;Sichuan Academy of Forestry;
单  位:
LI Jia-man%HUANG Zhen%YANG Han-bo%CHEN Zhi%XING Wen-xi%JIN Wei%GUO Hong-ying%Sichuan Academy of Forestry
关键词:
C.longepaniculatum N.Chao ex H.W.Li;;Tissue culture;;Culture medium;;Exogenous hormone
摘  要:
The tissue culture technique was selected for Cinnamomum longepaniculatum to breed excellent nursery stock, by analyzing and comparing the influence of disinfectant varieties, concentration and action time, MS medium, 6-BA, NAA and IBA on tissue culture of annual shoots. The results showed that contamination rates were no higher than 15% and survival rates were higher than 70%, under 10% of 84 disinfectant treatment for 15 min, or 15% of 84 disinfectant treatment for 10 min. The optimum medium for adventitious bud regeneration was sucrose 30 g·L~(-1)+carrageenan 6 g·L~(-1)+MS+6-BA 1.0 mg·L~(-1)+ IBA 0.1 mg·L~(-1); The medium for multiplication of adventitious buds was sucrose 30 g·L~(-1)+carrageenan 6 g·L~(-1)+MS+6-BA 1.0 mg·L~(-1)+NAA 0.10 mg·L~(-1)+IBA 0.2 mg·L~(-1); The rooting medium was sucrose 15 g·L~(-1)+carrageenan 6 g·L~(-1)+1/2 MS+IBA 1.5 mg·L~(-1)+NAA 1.0 mg·L~(-1).

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