作 者：
张美芳;何玲;张美丽;苑希蕊;杜方梦;赵凯茜
关键词：
‘秦美’猕猴桃;采后病原真菌;分离;鉴定
摘 要：
以‘秦美’猕猴桃为试材,分离纯化在贮藏过程中引起腐烂的主要病原真菌。采用形态学观察及核糖体rDNA-ITS(Internal transcribed spacer)区序列分析法进行鉴定。结果表明:从腐烂猕猴桃中共分离出5株致病菌,对ITS区序列测序结果经GenBank数据库BLAST比对,A的ITS序列与Trichothecium roseum(EU552162.1)同一分支,支持率达100%;B的ITS序列与Fusarium tricinctum(AB587078.1)同一分支,支持率达99%;C的ITS序列与Penicillium expansum(AF330635.1)同一分支,支持率达99%;D的ITS序列与Colletotrichum boninense(KF819619.1)同一分支,支持率达99%;E的ITS序列与Botrytis elliptica(KJ638600.1)同一分支,支持率达100%。由形态学及ITS区序列分析,构建系统发育树,最终鉴定A为粉红聚端孢(Trichothecium roseum),B为镰刀菌属(Fusarium tricinctum),C为扩展青霉(Penicillium expansum),D为炭疽病菌(Colletotrichum boninense),E为灰葡萄孢(Botrytis cinerea)。5种病原真菌中灰葡萄孢为主要致病菌。
译 名：
Identification of Pathogenic Fungi from Qinmei Kiwifruit at Stage of Post-harvest Storage
作 者：
ZHANG Meifang;HE Ling;ZHANG Meili;YUAN Xirui;DU Fangmeng;ZHAO Kaiqian;College of Horticulture,Northwest A&F University;
关键词：
Qinmei kivifruit;;Postharvest pathogen fungi;;Isolation;;Identification
摘 要：
With Qinmei kiwifruit as test material,the main pathogenic fungal were separated and sublimated from rot kiwifruit during it was stored.It was identified by sequence analysis of morphological and ribosomal rDNA-Internal transcribed spacer(ITS).Five pathogens was isolated from the rotten kiwifruit,then the results of the sequencing of ITS area were tested by using blast in GenBank database.The analysisof the sequence of A gene revealed 100% identity with Trichothecium roseum(EU552162.1);B gene revealed 99%identity with Fusarium tricinctum(AB587078.1);C gene revealed 99%identity with Penicillium expansum(AF330635.1);D gene revealed 99%identity with Colletotrichum boninense(KF819619.1);E gene revealed 100% identity with Botrytis elliptica(KJ638600.1).By sequence analysis of morphological and TTS,five pathogenic bacteria were designated as Trichothecium roseum,Fusarium tricinctum,Penicillium expansum,Colletotrichum boninense and Botrytis elliptica.Botrytis ellipticais the main pathogenic bacterias.
