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Position: Home > Articles > An Efficient Method for Preparation of the Total RNA from the Root of Mangrove Acta Agriculturae Boreali-Sinica 2009,24 (2) 19-21

一种有效的红树根部总RNA提取方法

作  者:
符秀梅;韩淑梅;朱红林;周秀;李小靖;吴辉;谢俊;陈银华
单  位:
海南大学农学院;海南大学教育部热带生物资源重点实验室
关键词:
红树;RNA提取;CTAB法
摘  要:
红树作为一种重要的耐盐植物,为耐盐研究提供了重要的基因资源。本研究分别采用SDS、TR IZOL、CTAB等3种不同的方法进行了红树RNA提取,经电泳分析、紫外吸收检测、cDNA合成等技术手段检测表明,改进的CTAB法所提取的红树总RNA质量最好。
译  名:
An Efficient Method for Preparation of the Total RNA from the Root of Mangrove
作  者:
FU Xiu-mei2,HAN Shu-mei2,ZHU Hong-lin2,ZHOU Xiu2,LI Xiao-jing2,WU hui2,XIE Jun2,CHEN Yin-hua1,2(1.Key Laboratory for Tropical Biological Resources,Ministry of Education,Haikou 570228,China;2.College of Agronomy,Hainan University,Haikou 570228,China)
关键词:
Mangrove;Preparation of RNA;Method of CTAB
摘  要:
The mangrove is an important salt-tolerance plant.In this research,three method for isolation of total RNA from the root of mangrove were done.The improved method of CTAB was verified to be the most efficient method through the way of the electrophoretic patterns of nucleic acids and absorbance at 230,260 and 280 nm in a UV-Vis spectrophotometer.

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