中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 免疫共沉淀筛选牛卵泡CART相互作用蛋白的研究 畜牧兽医学报 2015,46 (12) 2169-2175
Position: Home > Articles > Screening Proteins Interacting with Cocaine-and Amphetamine-Regulated Transcript in Bovine Follicles by Co-immunoprecipitation Chinese Journal of Animal and Veterinary Sciences 2015,46 (12) 2169-2175

免疫共沉淀筛选牛卵泡CART相互作用蛋白的研究

作  者：
李鹏飞;孟金柱;刘岩;黄洋;陈建伟;姜晓龙;曹霞;姚晓磊;赵妙妙
单  位：
山西农业大学动物科技学院;山西农业大学生命科学学院
关键词：
牛;CART;免疫共沉淀;质谱分析;相互作用
摘  要：
旨在研究牛卵泡发育过程中可卡因-苯丙胺调节转录肽(Cocaine-and amphetamine-regulated transcript,CART)相互作用蛋白(Protein-protein interaction,PPI)。选择4头10月龄同期发情的健康母牛,B超检测卵泡达8~12mm,屠宰并采集、分离卵泡颗粒细胞(Granulosa cells,GCs)后,进行总蛋白提取,兔抗CART一抗沉淀CART及其相互作用蛋白,经Protein G-Agarose磁珠吸附分离,多次洗涤去除盐分和杂蛋白,洗脱蛋白复合体并酶解,LC-ESI-Q-TOF质谱分析(Mass spectrometry,MS)及数据库比对。结果显示:共鉴定蛋白质组分111个;经Cytoscape V3.1.0分析,有81个蛋白符合功能模块,并构建蛋白相互作用网络图;同时对相互作用蛋白进行功能富集性分析,共分为3大类34组:其中分子功能占11.73%,生物学过程占46.93%,细胞组分占41.34%。α2巨球蛋白(A2M)和波形蛋白(VIM)与CART相互作用的相关系数最大;CART与NALP1、SERPINH1和PDIA6具有负调控应答的功能,提示可能参与牛卵泡发育过程的调控。
译  名：
Screening Proteins Interacting with Cocaine-and Amphetamine-Regulated Transcript in Bovine Follicles by Co-immunoprecipitation
作  者：
LI Peng-fei;MENG Jin-zhu;LIU Yan;HUANG Yang;CHEN Jian-wei;JIANG Xiao-long;CAO Xia;YAO Xiao-lei;ZHAO Miao-miao;LLi-hua;College of Life Science,Shanxi Agricultural University;College of Animal Science and Veterinary Medicine,Shanxi Agricultural University;
关键词：
bovine;;CART;;co-immunoprecipitation;;mass spectrometry;;interaction
摘  要：
The study is focused on screening the proteins interacting with cocaine-and amphetamine-regulated transcript(CART)during follicle development in bovine.Four 10-month-old healthy cows,treated for estrus synchronization,were monitored by B-ultrasonic machine to find the ovarian follicles with the diameter of 8-12 mm,which were collected followed by cows being slaughtered.The granulosa cells(GCs)were isolated from those ovarian follicles and then total protein was extracted.CART and the binding protein were deposited by primary antibodies(Rabbit anti CART)using Protein G-Agarose magnetic beads,followed by salt and miscellaneous protein being removed by washing repeatedly.Protein complexes was analyzed by LC-ESI-Q-TOF mass spectrometry(MS)and alignment followed by being eluted and digested by trypsin.Results showed that 111 proteins were identified,in which 81 proteins were functional in conformity with the interaction network modules by Cytoscape V3.1.0analysis.For the function enrichment analysis,those 81 proteins were classified into 34 groups in 3 categories:molecular function(11.73%),biological processes(46.93%),cell component(41.34%).The correlation coefficient of the interaction between A2 M,VIM and CART was maximum.It was also found CART was negatively regulated by NALP1,SERPINH1 and PDIA6,which suggested that they might participate in regulating follicle development in bovine.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46