Correlation analysis of sheep Myf5 gene polymorphism and carcass traits

REN Sirui;REN Tingting;HU Huiyu;LIU Mingjun;LI Wenrong;ZHANG Ning;ZHANG Xuemei;LIU Chenxi;HE Sangang;Institute of Biotechnology/Key Laboratory of Genetic Breeding and Reproduction of Herbivorous Livestock, Ministry of Agriculture and Rural Affairs/Xinjiang Key Laboratory of Animal Biotechnology, Xinjiang Academy of Animal Science;College of Animal Science, Xinjiang Agricultural University;

Texel ×Kazakh cross F2 generation sheep;;Myf5 gene;;SNPs;;carcass traits;;correlation analysis

In order to study the relationship between single nucleotide polymorphisms(SNPs) of Myf5 gene and carcass traits of Texel×Kazakh cross F2 generation sheep, PCR sequencing technology and time-of-flight mass spectrometry detection method were used to type Myf5 gene SNPs in 434 Texel×Kazakh cross F2 generation sheeps, and correlation analysis was conducted between the classification results and its carcass traits(carcass weight, carcass length, ankle width, hip width, chest width, shoulder width and chest depth).Results showed that a total of five SNPs loci were found in the Myf5 gene, namely A116460176 G, rs604841316, rs416158998, rs588855095 and rs412277497. Among them, rs416158998, an intron mutation, was moderately polymorphic in Texel×Kazakh cross F2 generation sheep(0.250.05), and significantly correlated with hip width(P<0.05). rs412277497, a missense mutation with serine mutated to cystein on the exon, was moderately polymorphic in sheep(0.250.05), and significantly or extremelly significantly correlated with carcass length, chest width and shoulder width(P<0.05 or P<0.01). A116460176 G和rs604841316 was not significantly correlated with carcass traits(P>0.05).The experiment revealed that rs416158998, rs588855095 and rs412277497 can provide reference genetic markers for breeding new breeds of sheep.