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Position: Home > Articles > Extraction of Antioxidant Active Components of Crotalaria mucronata Desv. and Optimization of Activity Determination System Journal of Anhui Agricultural Sciences 2010,38 (28) 15571-15573

猪屎豆抗氧化活性成分的提取及活性测定体系的优化

作  者:
姜佩佩;纪明慧;舒火明;李渊
单  位:
海南师范大学
关键词:
猪屎豆;抗氧化;鲁米诺
摘  要:
[目的]研究猪屎豆(Crotalaria mucronata Desv.)乙醇提取物的抗氧化活性。[方法]采用超声波辅助乙醇提取法提取猪屎豆抗氧化活性成分,用H2O2-鲁米诺化学发光体系测定清除羟自由基(·OH)的效率。并对测定体系进行优化。[结果]在料液比为3.6∶1,乙醇体积分数为70%,提取时间为30min,提取温度为80℃,猪屎豆乙醇提取物对·OH的清除效果最佳。清除率测定体系中,H2O2浓度为0.60%和Luminol浓度为1.00×10-4mol/L时测定结果较准确。[结论]猪屎豆乙醇提取物对·OH有很好的清除作用。
译  名:
Extraction of Antioxidant Active Components of Crotalaria mucronata Desv. and Optimization of Activity Determination System
作  者:
JIANG Pei-pei et al (Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education,Hainan Normal University,Haikou, Hainan 571158)
关键词:
Crotalaria mucronata Desv.; Antioxidation; Luminol
摘  要:
[Objective]The aim is to study the antioxidant activity of ethanol extracts from Crotalaria mucronata Desv..[Method]The ultrasonic-assisted ethanol extraction method is adopted to extract the antioxidant active components of crotalaria mucronata Desv,chemilumines-cence analysis in H2O2-Luminol system is used to determine efficiency of scavenging hydroxyl free radical and optimize the measurement system.[Result]Under the conditions that solid-to-liquid ratio is 3.6∶1,ethanol concentration is 70% ,extraction time is 30 min and temperature is 80 ℃,the test results are more accurate and crotalaria mucronata Desv.Shows the best scavenging effects on hydroxyl free radical.[Conclusion]The ethanol extract of Crotalaria mucronata Desv..Shows good cavenging effects of hydroxyl free radical.

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