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当前位置: 首页 > 文章 > 中甸牦牛mtDNA Cytb基因遗传多样性及系统进化分析 中国畜牧兽医 2016,43 (10) 2680-2687
Position: Home > Articles > Analysis on Genetic Diversity and Phyletic Evolution of mtDNA Cytb Gene in Zhongdian Yak China Animal Husbandry & Veterinary Medicine 2016,43 (10) 2680-2687

中甸牦牛mtDNA Cytb基因遗传多样性及系统进化分析

作  者：
涂世英;宋娜娜;柴志欣;钟金城
单  位：
云南省动物卫生监督所;西南民族大学
关键词：
中甸牦牛;细胞色素b基因;系统进化;遗传多样性
摘  要：
为从分子水平上探究中甸牦牛的遗传多样性和起源进化关系,本研究通过测定和分析中甸牦牛15个个体细胞色素b基因(Cytb)全序列,分析多态性及构建系统进化树。结果表明,中甸牦牛Cytb基因全序列长度为1 140bp,T、A、G和C含量分别为26.3%、31.8%、13.1%和28.8%;在15个个体中鉴定出3种单倍型,3个变异位点,其中2个转换、1个颠换,单倍型多样性(Hd)为0.2571,核苷酸多样性(Pi)为0.00035。系统进化树中,中甸牦牛首先与野牦牛聚为一类,之后与美洲野牛聚为一类,说明中甸牦牛与野牦牛和美洲野牛的亲缘关系较近、遗传相似性高,而与其他牛属的遗传相似性相对较低。遗传距离分析结果发现,中甸牦牛和野牦牛的遗传距离最近,结合分子生物学、古生物学等学科知识进一步支持了牦牛和野牦牛划为牛亚科牦牛属的观点。
译  名：
Analysis on Genetic Diversity and Phyletic Evolution of mtDNA Cytb Gene in Zhongdian Yak
作  者：
TU Shi-ying;SONG Na-na;CHAI Zhi-xin;ZHONG Jin-cheng;Animal Health Supervision Authority of Yunnan;Key Laboratory of Animal Genetics and Breeding of State Ethnic Affairs Commission and Ministry of Education,Southwest University for Nationalities;Institute of Tibetan Plateau Research,Southwest University for Nationalities;
关键词：
Zhongdian yak;;Cytb gene;;phylogenetic evolution;;genetic diversity
摘  要：
In order to investigate the genetic diversity and the origin of evolutionary relationship of Zhongdian yak,we analyzed the complete sequence of 15 individuals Cytb gene,its sequence polymorphism was analyzed,and the phylogenetic tree was constructed.The results showed that the length of the nucleotide sequence were 1 140 bp,with nucleotide frequencies of 26.3%,31.8%,13.1% and 28.8%for T,A,G and C,respectively.Three haplotypes were identified of 15 individuals,with 3polymorphic sites,including two conversions,one transversion,haplotype diversity was 0.2571 and nucleotide diversity was 0.00035.Phylogenetic analysis suggested that Zhongdian yak and Bos mutusc clustered firstly,then gathered with Bisonbison,which indicated that there were high genetic similarity and closer genetic relationship,genetic similarity with other cattle genus was relatively low.Combining with the proof of molecular biology and paleontology,the result supported the point that Bos grunniens and Bos mutus were classified as an alone genus in Bovinae.

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