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Position: Home > Articles > A Preliminary Study on Activity Mechanism and Identification of Extract of Pharbitis purpurea Seeds against Tetranychus cinnabarinus Scientia Silvae Sinicae 2011,47 (11) 202-206

牵牛子杀螨活性物质鉴定及其作用机制初探

作  者:
杜娟;赵磊;师光禄;王有年;仝宝生
单  位:
内蒙古永业生物技术有限责任公司;农业部都市农业(北方)重点开放实验室
关键词:
朱砂叶螨;亚油酸甲酯;谷胱甘肽-S-转移酶;乙酰胆碱酯酶;单胺氧化酶;Ca2+-ATP酶;Na+,K+-ATP酶
摘  要:
朱砂叶螨(Tetranychus cinnabarinus)具有个体小、繁殖快、代数多、适应性强、为害重等特点,是世界公认的最难防治的有害生物群落(Hazan et al.,1974;何林等,2004),可危害百余种农作物和果树,并对其产量和品质造成严重的影响(Ho et al.,1997)。长期以来,大量重复使用非专一性化学杀螨剂,使许多螨类已产生抗药性(唐除痴等,1998),并且对杀螨剂出现了交互抗性(王旭等,1997),与
译  名:
A Preliminary Study on Activity Mechanism and Identification of Extract of Pharbitis purpurea Seeds against Tetranychus cinnabarinus
作  者:
Du Juan1 Zhao Lei1 Shi Guanglu1 Wang Younian1 Tong Baosheng2 (1.Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture ; College of Biotechnology, Beijing University of Agriculture Beijing 102206; 2.Inner Mongolia Yongye Biotechnology Co., Ltd. Huhhot 010010)
关键词:
Tetranychus cinnabarinus; methyl linoleate; glutathione-S-transferase; acetylcholine esterase; mono-amine oxidase; Ca2+-ATPase; Na+,K+-ATPase
摘  要:
Fraction 8 of the extractants from semen pharbitidis was identified and its mechanism of biological activity against Tetranychus cinnabarinus was investigated. The main component of the extractant fraction 8 was detected with nuclear magnetic resonance (NMR), gas chromatography and mass spectrum (GC-MS). Influences of the main component on several enzymes activities such as glutathione-S-transferase (GSTs), acetylcholine esterase (AChE) and mono-amine oxidase (MAO), in T. cinnabarinus body were detected by using biochemical method. Results showed that the main component of the extractant fraction 8 was methyl linoleate. The methyl linoleate activated glutathione-S-transferase in T. cinnabarinus body, while it inhibited to various degrees acetylcholine esterase and mono-amine oxidase related to nervous system, and hence caused the death of T. cinnabarinus. Therefore the methyl linoleate has a good prospect developing into new pesticide.

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