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当前位置: 首页 > 文章 > cGAS蛋白促进PCV-2茎环结构诱导cGAS-STING信号通路天然免疫反应研究 黑龙江畜牧兽医 2024 (13) 59-65,71
Position: Home > Articles > Research on cGAS promoted PCV-2 stem-loop structure to induce the innate immune response in the cGAS-STING signaling pathway Heilongjiang Animal Science and Veterinary Medicine 2024 (13) 59-65,71

cGAS蛋白促进PCV-2茎环结构诱导cGAS-STING信号通路天然免疫反应研究

作  者：
苏冰;邓治邦
单  位：
湖南农业大学动物医学院
关键词：
猪圆环病毒2型;茎环结构;环鸟苷酸腺苷酸合成酶;cGAS-STING信号通路;天然免疫反应
摘  要：
为了探索猪圆环病毒2型(PCV-2)DNA茎环结构是否可以激活环鸟苷酸腺苷酸合成酶(cGAS),以及是否诱导cGAS-STING信号通路天然免疫反应,试验将含有PCV-2茎环结构的单链DNA(SL-DNA)转染至PK15细胞中,通过Western-blot检测cGAS蛋白是否在PK15细胞中表达;将SL-DNA转染至过表达及敲除cGAS蛋白的PK15细胞中,通过实时荧光定量PCR检测细胞中cGAS、干扰素刺激基因(STING)、TANK结合激酶1(TBK1)、干扰素调节因子3(IRF3)、干扰素-β(IFN-β)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)基因的表达水平.结果表明:SL-DNA转染后,PK15细胞中cGAS蛋白表达量上升;过表达cGAS蛋白的PK15细胞与PK15细胞相比,SL-DNA转染后cGAS、STING、TBK1、IRF3、IFN-β、IL-1β、IL-6、TNF-α基因的相对表达量均极显著升高(P<0.01);敲除cGAS蛋白的PK15细胞与PK15细胞相比,SL-DNA转染后cGAS、STING、TBK1、IRF3、IFN-β、IL-1β、IL-6、TNF-α基因的相对表达量均极显著降低(P<0.01).说明cGAS蛋白可以识别PCV-2的茎环结构,并且促进其诱导的cGAS-STING信号通路天然免疫反应.
译  名：
Research on cGAS promoted PCV-2 stem-loop structure to induce the innate immune response in the cGAS-STING signaling pathway
单  位：
College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China
关键词：
Porcine circovirus type 2(PCV-2)%stem-loop structure%cyclic guanosine adenylate synthase%cGAS-STING signaling pathway%innate immune response
摘  要：
In order to explore whether Porcine circovirus type 2(PCV-2)DNA stem-loop structure could activate cyclic guanosine adenylate synthase(cGAS)and whether it could induce a natural immune response to the cGAS-STING signaling pathway,in the test,single-stranded DNA(SL-DNA)containing PCV-2 stem-loop structure was transfected into PK15 cells,and whether cGAS was expressed in PK15 cells was detected by Western-blot.SL-DNA was transfected into cGAS-overexpressing and cGAS-knockout PK15 cells,and the gene expression levels of cGAS,interferon-stimulated gene(STING),TANK-binding kinase 1(TBK1),interferon-regulatory factor 3(IRF3),interferon-beta(IFN-β),interleukin-1beta(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)were detected in the cells by realtime fluorescence quantitative PCR.The results showed that cGAS expression increased in PK15 cells after SL-DNA transfection.The relative gene expression of cGAS,STING,TBK1,IRF3,IFN-β,IL-1β,IL-6,and TNF-αwas extremely significantly elevated in cGAS-overexpressing PK15 cells compared with PK15 cells after SL-DNA transfection(P<0.01).The relative expression of extremely cGAS,STING,TBK1,IRF3,IFN-β,IL-1β,IL-6,and TNF-αgenes were all extremely significantly decreased(P<0.01)in cGAS-knockout PK15 cells compared with PK15 cells after SL-DNA transfection.The results suggested that cGAS recognized the stem-loop structure of PCV-2 and promoted its induced natural immune response by the cGAS-STING signaling pathway.

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