关键词：
β-D-呋喃果糖苷酶;离子交换法;固定化;响应面法
摘 要：
以大孔阴离子树脂D311为载体,对日本曲霉来源的β-D-呋喃果糖苷酶进行离子交换法固定化。研究温度、pH值、时间、游离酶液酶活力对固定化效果的影响,并在此基础上运用响应面法对固定化条件进行优化。结果表明,最佳固定化条件为:室温、pH6.6、固定化时间4h、游离酶液酶活力为900U/mL,在此条件下,固定化β-D-呋喃果糖苷酶生产的低聚果糖产量可达58.16%。
译 名：
Optimization of Ion Exchange Immobilization of β-Fructofuranosidase Using Response Surface Methodology
作 者：
ZHANG Yuan-yuan,NIE Shao-ping,WAN Cheng,XIE Ming-yong (State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China)
关键词：
β-fructofuranosidase;ion exchange adsorption;immobilization;response surface methodology (RSM)
摘 要：
D311 type macroporous anion exchange resin was used as a carrier for the immobilization of β-fructofuranosidase derived from Aspergillus japonicus. The effects of immobilization time and temperature,pH and enzyme activity of free β-fructofuranosidase solution on the catalytic ability of immobilized β-fructofuranosidase were investigated by single factor method and based on this,except immobilization temperature,three other conditions were optimized using response surface methodology. The optimum immobilization conditions were determined as follows:enzyme activity of free β-fructofuranosidase solution 900 U/mL and pH 6.6 for 4 h immobilization at room temperature,and the yield of fructooligosaccharides using immobilized β-fructofuranosidase under these conditions was 58.16%.
