当前位置: 首页 > 文章 > 大豆疫霉的土壤诱集检测 粮食储藏 1998 (5) 8-14
Position: Home > Articles > DETECTION OF PHYTOPHTHORA SOJAE FROM SOIL WITH SOYBEAN LEAF-DISC BAITING Grain Storage 1998 (5) 8-14

大豆疫霉的土壤诱集检测

作  者:
彭金火
单  位:
大连动植物检疫局
关键词:
大豆疫霉;土壤检测;叶碟诱集;选择性诱集
摘  要:
采用大豆叶碟诱集的方法研究了影响大豆疫霉(PhytophthorasojaeKaufmann&Gerdemann)土壤检测的因素。结果表明,自然感病的风干土壤加蒸馏水湿润至饱和或接近饱和状态,光照条件下预培养4~6d,加5~10mm深的蒸馏水浸泡,然后加感病大豆品种的叶碟诱集6~24h.可以较稳定地检测到大豆疫霉。叶碟诱集后用蒸馏水培养或用选择性培养基培养的检测效果相似,但用蒸馏水培养便于检查检测结果。当诱集用的离体叶碟采自含抗病基因的大豆品种的植株时,只有能克服该基因的大豆疫霉能被诱集和检测到。
译  名:
DETECTION OF PHYTOPHTHORA SOJAE FROM SOIL WITH SOYBEAN LEAF-DISC BAITING
作  者:
Peng Jinhuo(Dalian Animal and Plant Quarantine Bureau. Dalian, PRC, 116001)Terry Anderson(Greenhouse and Processing Crops Research Centre. Harrow. Ontario NOR 1Go, Canada)
关键词:
Phytophthora sojae, soil detection, leaf-disc baiting,selective baiting
摘  要:
The factors concerning of the detection of Phytophthora sojae from soil with soybean leafdisc baiting method were studied. High detection rate of flask and leaf-disc infection ofP. sojae were obtained, when air -dried, naturally infected soybean field soils in flaskwere moistened and pre-incubated under lights at 22~26℃ for 4~6 days, then floodedwith 5~10mm depth excess distilled water, and then baited with soybean leaf-discs for6~24 hours. Distilled water, other than selective medium. was adopted to incubate thebaited leaf-discs. which proved a sensitive technique for the detection of P. sojae. Whenbaiting leaf-discs came from a plant with special resistant gene, only those pathogenswhich can overcome this gene can be baited and detected.

相似文章

计量
文章访问数: 19
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊