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Position: Home > Articles > Electroantennographic and behavioral responses of Anomala corpulenta to plant volatiles Plant Protection 2015,41 (1) 33-38

铜绿丽龟对植物源挥发物的触角电位和行为反应

作  者:
谢明惠;陈浩梁;衣建坤;李克斌;张杰;苏卫华
单  位:
安徽省农业科学院植物保护与农产品质量安全研究所;中国农业科学院植物保护研究所
关键词:
铜绿丽金龟;植物源挥发物;Y型嗅觉仪;触角电位技术;行为反应
摘  要:
为筛选适宜的铜绿丽金龟植物源引诱剂,利用触角电位(EAG)和Y形嗅觉仪技术,测定了铜绿丽金龟雌、雄虫对20种植物源挥发物的电生理和行为反应。结果表明:在10 μg/μL浓度下,铜绿丽金龟对甲基庚烯酮、顺-3-己烯乙酸酯、壬醛、1-辛烯-3-醇、芳樟醇和反-2-己烯醇具有较强的触角电位反应,且呈雌虫大于雄虫的趋势。Y形嗅觉仪行为测定中,雌虫对多数挥发物的敏感度高于雄虫。其中反-2-己烯醇、顺-3-己烯乙酸酯、甲基庚烯酮和1-辛烯-3-醇对铜绿丽金龟雌虫的吸引作用显著高于对照;而雄虫仅对顺-3-己烯乙酸酯有显著的趋向选择。本研究为开发有效的铜绿丽金龟田间引诱剂用于生态治理提供依据。
译  名:
Electroantennographic and behavioral responses of Anomala corpulenta to plant volatiles
作  者:
谢明惠(1);;陈浩梁(2);;衣建坤(1);;李克斌(1);;张杰(1);;苏卫华(2)
单  位:
(1)中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京, 100193;;(2)安徽省农业科学院植物保护与农产品质量安全研究所, 合肥, 230031
摘  要:
To find possible attractants, 20 kinds of plant volatiles were chosen for electroantennogram test and Y-tube olfactometer bioassays using both female and male Anomala corpulenta. The results showed that methyl heptenone,cis-3-hexenyl acetate, nonanal, l-octene-3-alcohol, linalool and trans-2-hexenol elicited significant EAG responses at the concentration of 10 μg/μL; furthermore, the response of females was stronger than that of males. The Y-tube olfactometer bioassays indicated that females were more sensitive to most volatiles than males. Compared with the control, females were attracted by trans-2-hexenol, cis-3-hexenyl acetate, 6-methyl 5-hepten-2-one and l-octene-3-alcohol, but males were only attracted by cis-3-hexenyl acetate. This study could provide a theoretical basis for developing attractants of A. corpulenta for ecological management.

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