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HPLC测定复方磺胺间甲氧嘧啶钠可溶性粉含量

作  者:
杨武宁;崔艳莉
单  位:
广西兽药监察所
关键词:
高效液相色谱法;磺胺间甲氧嘧啶;甲氧苄啶;复方磺胺间甲氧嘧啶钠可溶性粉
摘  要:
建立复方磺胺间甲氧嘧啶钠可溶性粉中磺胺间甲氧嘧啶钠(以磺胺间甲氧嘧啶计)和甲氧苄啶含量的HPLC检测方法,采用Inertsil C18色谱柱(4.6mm×250mm,5μm),以乙腈:0.017mol/L磷酸液(20:80)(按1‰的比例加入三乙胺)为流动相,流速为1mL/min,检测波长为230nm。在该色谱条件下,两种药物分离很好,磺胺间甲氧嘧啶在24.89~248.9μg/mL之间,甲氧苄啶在5~50μg/mL之间,线性良好(r>0.99999),添加回收率在97.46%~100.45%之间。该方法快速简便,准确可靠,能够满足实际样品的检测要求。
译  名:
HPLC Determination for Sulfamonomethoxine and TMP in Compound Sulfamonomethoxine Sodium Soluble Powder
作  者:
Yang Wuning,Cui Yanli* (Guangxi Institute of Veterinary Drug Control,Nanning,Guangxi, 530001)
关键词:
HPLC; sulfamonomethoxine; TMP; compound sulfamonomethoxine sodium soluble powder
摘  要:
To establish an HPLC method for determination of sulfamonomethoxine and TMP in Compound sulfa-monomethoxine sodium soluble powder. Sulfamonomethoxine and TMP in the soluble powder were determined by HPLC with a Inertsil C18 column (4.6mm×250mm,5μm)with UV detection at 230nm, and the mobile phase was consisted of acetonitrile-0.017mol/L phosphoric acid(20:80,)(the proportion of 1 ‰ to join triethylamine). The flow rate was 1.0ml/min. Sulfamonomethoxine and TMP in compound sulfamonomethoxine sodium soluble powder can be separated well under the chromatographic condition. The calibration curve was linear (r > 0.99999)within the range of 24.89~248.9μg/mL for sulfamonomethoxine. It the range of 5~50μg/mL for TMP, the recovery was 97.46%~100.45%.The method is simple, quick, accurate and can be meet the detection requirements.

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