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当前位置: 首页 > 文章 > 褪黑素对大鼠纤维化TGF-β_1/Smads信号通路干预研究 黑龙江农业科学 2019 (1) 58-61
Position: Home > Articles > Intervention Study of Melatonin on TGF-β_1/Smads Signaling Pathway in Rat Fibrosis Heilongjiang Agricultural Sciences 2019 (1) 58-61

褪黑素对大鼠纤维化TGF-β_1/Smads信号通路干预研究

作  者：
孙旭;张哲;孙琛;张辉;温巨明;丁海麦
单  位：
包头医学院基础医学与法医学院;包头医学院乌兰察布临床学院
关键词：
褪黑素;肾间质纤维化;TGF-β1;Smad3;Smad7
摘  要：
褪黑素是一种主要由松果体分泌的多效性神经激素,具有多种生物学功能,包括抗氧化应激、抗炎、抗凋亡和抗肿瘤特性,以及调节昼夜节律。为探寻临床治疗肾间质纤维化的新途径,本试验观察褪黑素对单侧输尿管梗阻(UUO)构建大鼠肾纤维化模型TGF-β_1/Smads信号通路影响,探讨其干预对肾纤维化机制,SD大鼠随机分为假手术(Sham)组、UUO模型(UUO)组、UUO+5mg·kg~(-1)褪黑素组、UUO+10mg·kg~(-1)褪黑素组、UUO+15mg·kg~(-1)褪黑素组,每组6只。均于手术当天晚上灌胃给药,每24h1次。14d处死大鼠,取血清检测血肌酐、尿素氮,肾组织进行Masson染色,观察病理结果,实时定量PCR检测肾组织TGF-β_1、Smad3、Smad7和Collagen Type I mRNA表达。结果表明:褪黑素减轻肾组织纤维化病理损伤,使肾组织TGF-β_1、Smad3、Collagen Type I mRNA表达降低,Smad7mRNA表达升高(P<0.05,部分P<0.01),以15mg·kg~(-1)改善效果最佳。褪黑素可呈剂量相关性下调TGF-β_1、Smad3、Collagen Type I及上调Smad7来抑制TGF-β_1/Smads信号通路,从而发挥肾间质纤维化作用。
译  名：
Intervention Study of Melatonin on TGF-β_1/Smads Signaling Pathway in Rat Fibrosis
作  者：
SUN Xu;ZHANG Zhe;SUN Chen;ZHANG Hui;WEN Ju-ming;DING Hai-mai;Wulanchabu Clinical College,Baotou Medical College;Department of Biochemistry and Molecular Biology,Baotou Medical College;
单  位：
SUN Xu%ZHANG Zhe%SUN Chen%ZHANG Hui%WEN Ju-ming%DING Hai-mai%Wulanchabu Clinical College,Baotou Medical College%Department of Biochemistry and Molecular Biology,Baotou Medical College
关键词：
melatonin;;renal interstitial fibrosis;;TGF-β1;;Smad3;;Smad7
摘  要：
Melatonin is a pleiotropic neurohormone secreted mainly by the pineal gland.It has a variety of biological functions including anti-oxidative stress,anti-inflammatory,anti-apoptotic and anti-tumor properties,as well as regulation of circadian rhythm.In order to explore a new way to prevent renal interstitial fibrosis.In this study we observed the effect of melatonin on the unilateral ureteral obstruction(UUO)constructing the renal fibrosis model,SD rats were randomly divided into sham operation group,UUO model(UUO)group,UUO + 5mg·kg~(-1) melatonin group,UUO +10mg·kg~(-1) melatonin group,UUO + 15mg·kg~(-1) melatonin group,each group 6.They were administered by intragastric administration on the evening of the operation,once every 24 hours.The rats were sacrificed for 14 days.Serum creatinine and urea nitrogen were detected by serum.Masson staining was used to observe the pathological results of kidney tissue.The expressions of TGF-β_1,Smad3,Smad7 and Collagen Type I mRNA were detected by real-time quantitative PCR.The results showed that melatonin reduced the pathological damage of renal fibrosis,decreased the expression of TGF-β_1,Smad3 and Collagen Type I mRNA,and increased the expression of Smad7 mRNA(P<0.05,part P<0.01)to 15mg·kg~(-1) improves best.Melatonin can down-regulate TGF-β_1,Smad3,Collagen Type I and up-regulate Smad7 to inhibit TGF-β_1/Smads signaling pathway,thereby exerting renal interstitial fibrosis.

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