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Position: Home > Articles > Transformation of Human Bronchial Epithelial Cells Induced by Captan Agrochemicals 2011,50 (4) 35-39+46

克菌丹诱导人支气管上皮细胞转化

作  者:
许建宁;董琳;王全凯;胡洁;李学峰
单  位:
中国疾病预防控制中心职业卫生与中毒控制所;中国农业大学理学院
关键词:
克菌丹;人支气管上皮细胞;细胞转化
摘  要:
[方法]16HBE细胞经0.0625~1.0 mg/L剂量的克菌丹染毒,通过观察染毒细胞形态及恶性表型鉴定,检测克菌丹诱导16HBE细胞的转化。[结果]克菌丹转化第30代细胞生长速度增快,对conA凝集敏感性增强,失去贴壁生长依赖性,电镜下可见明显的形态学改变,裸鼠体内形成肿块为鳞状上皮细胞癌。[结论]克菌丹可直接诱导16HBE细胞发生恶性转化,该模型的建立为克菌丹对健康损伤效应研究及其评价奠定了基础。
译  名:
Transformation of Human Bronchial Epithelial Cells Induced by Captan
作  者:
XU Jian-ning1,2,DONG Lin2,WANG Quan-kai2,HU Jie2,LI Xue-feng1(1.college of science,china Agricultural University,Beijing 100094,china;2.chinese center for Disease control and Prevention National institute of occupational health and Poison control,Key laboratory of chemical safety and health,chinese center for Disease control and Prevention,Beijing 100050,china)
关键词:
captan;human bronchial epithelial cell;cell transformation
摘  要:
[Methods] 16hBe cell was treated with captan at the concentration from 0.0625 to 1.0 mg/l and observed biological characteristics to ensure whether cell malignant transformation occurred.[Results] The 30th generation of 16hBe treated with captan grew faster,while its sensibility to conA and ability of growing in semi-solid agar also increased.The morphological change of transformed cells could be founded under scanning electron microscope and the pathology suggested that tumor formed in nude mice was squamous cell carcinoma.[Conclusions] captan could induce 16hBe cell to get malignant transformation.This transformation model would be the foundation for further studying and evaluating the health damage caused by captan.

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