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Position: Home > Articles > Determination of Chestnut Polysaccharide Content with Anthrone-sulfuric Acid Method Journal of Hebei Normal University of Science & Technology 2010,24 (2) 57-62

蒽酮—硫酸法测定板栗多糖含量

作者：

李润丰;常学东;狄小丽

单位：

河北科技师范学院食品科技学院

关键词：

板栗多糖;蒽酮—硫酸法;测定

摘要：

采用蒽酮—硫酸法测定板栗多糖含量并对其测定条件进行优化。结果表明,蒽酮—硫酸法测定板栗多糖的适宜操作条件为:加入2g/L蒽酮—硫酸溶液4mL,在沸水浴中加热3min,自来水冷却40min后在10min内于波长600nm下读取吸光度。蒽酮—硫酸溶液需要现配现用。多糖含量控制在0.02～0.06g/L之间为宜。蒽酮—硫酸法测定板栗多糖含量的精密度实验RSD值为0.792%,平均加样回收率为107.2%,RSD值为4.6%。用本方法测定板栗中多糖含量为12.67%。

译名：

Determination of Chestnut Polysaccharide Content with Anthrone-sulfuric Acid Method

作者：

LI Run-feng,CHANG Xue-dong,DI Xiao-li(College of Food Engineering,Hebei Normal University of Science & Technology,Qinhuangdao Hebei,066600,China)

关键词：

Chestnut polysaccharide;Anthrone-sulfuric method;determination

摘要：

The determination of chestnut polysaccharide content using anthrone-sulfuric acid method was studied and the operating conditions were optimized.The results showed that the optimal operating conditions were:4 mL of 0.2% anthrone-sulfuric acid solution,heated in boiling water bath for 3 min,cooled with city water for 40 min,read the absorbance under 600 nm wavelength in 10 min after cooling.The anthrone-sulfuric acid solution must be used quickly.Polysaccharide concentration between 0.02~0.06 g/L was the best for determination.The RSD in accuracy experiment was 0.792%,and the average recovery rate was 107.2% with RSD of 4.6%.The content of polysaccharide in chestnut determined by this method was 12.67%.

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