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当前位置: 首页 > 文章 > HPLC同时测定锦绣杜鹃四种黄酮类成分的含量 西北林学院学报 2013,28 (1) 119-122
Position: Home > Articles > Simultaneous Determination of Four Flavonoids in the Leaves of Rhododendron pulchrum by HPLC Journal of Northwest Forestry University 2013,28 (1) 119-122

HPLC同时测定锦绣杜鹃四种黄酮类成分的含量

作  者：
张梅;潘大仁
单  位：
福建农林大学生命科学学院;闽江学院化工系
关键词：
HPLC;锦绣杜鹃;黄酮类化合物;含量测定
摘  要：
在建立高效液相色谱法(HPLC)同时,测定锦绣杜鹃中金丝桃苷、异槲皮苷、槲皮苷、槲皮素4种黄酮类化合物的含量。采用Waters C18(4.6mm×250mm,5μm)反相色谱柱,甲醇(B)和水(A)为流动相,梯度洗脱程序为:0～24min,31%～42%B;24～30min,42%～50%B;30～35min,50%～60%B;35～40min,60%B;检测波长356nm,流速为0.7mL.min-1,柱温30℃。结果显示锦绣杜鹃中4种黄酮类化合物达到了较好的分离,其中平均回收率分别为97.73%、101.71%、98.70%、104.28%;RSD分别为2.25%、1.31%、1.82%、2.81%(n=5),说明该分析方法简便、准确、重现性好,适合于锦绣杜鹃中黄酮类化合物含量的测定,其中锦绣杜鹃中黄酮类成分在4月份达到最大值。
译  名：
Simultaneous Determination of Four Flavonoids in the Leaves of Rhododendron pulchrum by HPLC
作  者：
ZHANG Mei1,PAN Da-ren2﹡(1.Department of Chemistry and Chemical Engineering,Minjiang University,Fuzhou,Fujian 350108,China; 2.College of Life Science,Fujian Agricultural and Forestry Universities,Fuzhou,Fujian 350002,China)
关键词：
HPLC;Rhododendron pulchrum;flavonoids;content determination
摘  要：
The objective of this study was to establish a HPLC method for simultaneous determination of 4 kinds of flavonoids(quercetin-3-galactoside,quercetin-3-glucoside,quercitrin,and quercetin) in the leaves of Rhododendron pulchrum.The following system was found to be optimum for the analysis:Waters C18 column(4.6 mm×250 mm,5 μm) by gradient elution with mobile phase A(water) and B(methanol):0-24 min,31%-42% B;24-30 min,42%-50% B;30-35 min,50%-60% B;35-40 min,60% B.The detection wavelength was 356 nm,and the rate flow was 0.7 mL/min with column temperature at 30℃.Under these conditions,4 types of flavonoids could be well separated.The average recovery of 4 types of flavoniods(quercetin-3-galactoside,quercetin-3-glucoside,quercitrin,and quercetin) were 97.73%,101.71%,98.70% and 104.28%,and the relative standard deviations of the method were 2.25%,1.31%,1.82% and 2.81%(n=5),respectively.The method was accurate and sensitive,which could be used for the determination of flavoniods in the leaves of R.pulchrum.The contents of flavonoids were in the highest levels in april.

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