Detection of plant hormone abscisic acid using optical surface plasmon resonance biosensing technology

MU Lin-ying;WANG Man-ping;WANG Shun;WANG Ting-ting;CAO Bai-qiong;SUN Xiao-hui;ZHU Juan-hua;HU Jian-dong;College of Mechanical and Electrical Engineering,Henan Agricultural University;Henan Nongda Xunjie Measurement Technology Co. ,Ltd.;

abscisic acid;;surface plasmon resonance;;molecular recognition membrane;;biosensor;;label free detection

The use of portable optical bosensor based on the surface plasmon resonance( SPR) for rapid detection of the plant hormone abscisic acid( abscisic acid,ABA) is considered. A novel and label-free approach using detection of the specific binding reaction between the ABA antibody and corresponding antigen is established. The biological molecular recognition membrane is first prepared by immobilizing ABA antigen on the surface of SPR sensor chip,and the ABA concentration in standard sample is detected by employing competitive inhibition method. The performance capabilities of the method are discussed and illustrated by quantitative detection of ABA in the standard ABA samples at concentration of 0,25,50,200,500,1 000 mg·L-1,respectively. The experimental results show that a good line relationship between SPR response signals and ABA concentrations is obtained with the coefficient of correlation-0. 998. The repeatability indicated by the relative standard deviation( RSD) was 4. 13%.