Position: Home > Articles > Detection of Plasmid-mediated Quinolone-resistant Genes in Escherichia coli Isolated from Dogs and Analysis of Their Drug Sensitivity
Acta Agriculturae Jiangxi
2018
(4)
79-82
犬源大肠杆菌质粒介导的喹诺酮类药物耐药基因的检测及药物敏感性分析
作 者：
田亚凯
单 位：
河南农业大学牧医工程学院
关键词：
犬源大肠杆菌;质粒介导的喹诺酮类药物耐药基因;药物敏感性;PCR检测
摘 要：
采用PCR方法检测了102株犬源大肠杆菌中质粒介导的喹诺酮类药物耐药(plasmid-mediated quinolone resistance,PMQR)基因的分布,并采用微量肉汤稀释法测定了6种抗菌药物对犬源大肠杆菌的最小抑菌浓度(MIC)。在被检测的8种PMQR基因中,仅有oqx A、oqx B、qnr S这3种耐药基因被检测出,检出率分别为9.80%、11.76%和14.70%,其中同时含有2种耐药基因的菌株检出率为8.82%,同时含有3种耐药基因的菌株检出率为4.90%;犬源大肠杆菌对喹诺酮类药物已经具有较高的耐药性,其对恩诺沙星、环丙沙星和氧氟沙星的耐药率分别达到了71.57%、64.71%、48.04%。
译 名：
Detection of Plasmid-mediated Quinolone-resistant Genes in Escherichia coli Isolated from Dogs and Analysis of Their Drug Sensitivity
作 者：
TIAN Ya-kai;College of Veterinary Medicine Engineering,Henan Agricultural University;
关键词：
Escherichia coli isolated from dog;;Plasmid-mediated quinolone-resistant(PMQR) gene;;Drug sensitivity;;PCR detection
摘 要：
The distribution of plasmid-mediated quinolone-resistant( PMQR) genes in 102 strains of Escherichia coli isolated from dogs was detected by using PCR method,and the minimal inhibitory concentrations( MICs) of 6 kinds of antibiotics to dog-derived E. coli were measured by micro-broth dilution assay. Among the tested 8 kinds of PMQR genes,only oqx A,oqx B and qnr S were detected out from these dog-derived E. coli strains,and their detection rate was 9.8%,11.76% and 14.70%,respectively. The strains simultaneously containing 2 kinds of PMQR genes accounted for 8.82%,and the strains containing 3 kinds of PMQR genes at the same time accounted for 4.90%. These dog-derived E. coli strains had a stronger resistance( tolerance) to quinolone antibiotics,and their resistant( tolerant) rate to Enrofloxacin( ENR),Ciprofloxacin( CIP) and Ofloxacin( OFL)reached 71.57%,64.71% and 48.04%,separately.
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