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Sex Identification of Red-crowned Crane by the Polymerase Chain Reaction

作  者:
LI Jian-hong;LI Shu-ling;BAG Jun;BAI Xiu-juan
单  位:
;Life Science College, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC
关键词:
red-crowned crane;sex determining gene;sex identification
摘  要:
Sex determining gene primers of Oriental White Stork were used to amplify sex-linked gene of the Red-crowned Crane' s W chromosome-specific by PCR for sex identification. The sexes of 7 couples of grown Red-crowned Cranes and 15 youngs were identified. Through DNA sequence analysis, the identity is 94.77% between Red-crowned Crane and Oriental White Stork. The results of this study suggest that the application of the polymerase chain reaction technique is practicable for determining sex in the Red-crowned Crane.
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