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Position: Home > Articles > ON A LARGE SCALE ISOLATION OF TI PLASMID BY THF MODIFIED METHODS Acta Phytopathologica Sinica 1988 (4)

大量提取Ti质粒方法的改进

作  者:
李全义;王金生;方中达
单  位:
南京农业大学植物病原生物研究室;南京农业大学植物病原生物研究室 硕士研究生
摘  要:
综合Currier和Wester,Casse,Kado和White等人的方法,发展了二种大量提纯Ti质粒的改进方法。改进法Ⅰ类似于Casse法,仅涉及PEG(聚乙二醇)一次沉淀.改进法Ⅱ涉及PEG和乙醇两次沉淀。改进法的特点是利用Nacl和苯酚-氯仿结合去除蛋白及染色体DNA。其优点是重复性好,产量高,易于掌握.
译  名:
ON A LARGE SCALE ISOLATION OF TI PLASMID BY THF MODIFIED METHODS
作  者:
Li Quanyi Wang Jinshaeng Fang Chongtah(Nanjing Agricultural University)
摘  要:
Two modifeid methods for isolation of Ti plasmid have been developed,based on the methods described by Currier and Nester,Casse,Kado and White.The mixture of phenol-chloroform (1:1) and 0.5M NaCl have been used forremoval of proteins and chromosomal DNA in the modified methods.The twomethods have been proved to be very efficient in the isolation of Ti plasmidfrom Agrobacterium tumefaciens.

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