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当前位置: 首页 > 文章 > 牦牛胎儿皮肤毛囊的形态发生及E钙黏蛋白的表达和定位 畜牧兽医学报 2016,47 (2) 397-403
Position: Home > Articles > Fetal Skin Hair Follicle Morphogenesis and E-Cadherin Expression of the Yak Chinese Journal of Animal and Veterinary Sciences 2016,47 (2) 397-403

牦牛胎儿皮肤毛囊的形态发生及E钙黏蛋白的表达和定位

作  者：
佘平昌;梁春年;裴杰;褚敏;郭宪;阎萍
单  位：
中国农业科学院兰州畜牧与兽药研究所
关键词：
牦牛;毛囊;E-cadherin;形态发生;qRT-PCR;免疫组织化学
摘  要：
拟研究牦牛毛囊的形态发生过程及胎儿毛囊发生的起始部位,分析E-cadherin(CDH1)对毛囊发育的作用。采取牦牛胚胎头部皮肤制备组织切片,观察毛囊的形态发生过程;利用免疫组织化学技术,定位E-cadherin蛋白在毛囊中的表达;使用qRT-PCR方法,比较不同胎龄胎儿头部皮肤中E-cadherin mRNA转录水平。结果表明,毛囊黑色素颗粒在牦牛胎儿皮肤中不同部位先后聚集,牦牛胎儿头部皮肤在胎龄60~70d时初级毛囊开始形成毛芽,胎龄130d时形成毛球结构。次级毛囊在胎龄80~90d时从初级毛囊中分化出来;并且毛囊的发育可能最先是从头部开始的,其中唇部、眉毛、睫毛、角缘发育最明显;E-cadherin蛋白定位于头部皮肤的表皮、真皮及毛囊中,在表皮中表达较高,在毛囊中呈中等阳性表达;E-cadherin mRNA在牦牛不同胎龄皮肤毛囊中相对转录水平整体呈上升趋势,而在90d时转录量较低,显著低于70、120和130d时(P<0.05)。牦牛头部毛囊在60~70d时开始形成毛芽,毛囊发育的起始部位可能是从头部开始的。E-cadherin基因可能参与了牦牛毛囊的发育。
译  名：
Fetal Skin Hair Follicle Morphogenesis and E-Cadherin Expression of the Yak
作  者：
SHE Ping-chang;LIANG Chun-nian;PEI Jie;CHU Min;GUO Xian;YAN Ping;Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS;Key Laboratory of Yak Breeding Engineering of Gansu Province;
关键词：
yak;;hair follicle;;E-cadherin;;morphogenesis;;qRT-PCR;;immunohistochemistry
摘  要：
The objective of this study was to observe yak hair follicle morphogenesis and fetal hair follicle original sites,study the importance of E-cadherin(CDH1)in the development of hair follicles.Skin tissue sections of the Yak embryo head were observed,and the hair follicle morphogenesis was studied.The melanin granules of fetal skin at different month and the development of hair follicle original sites were detected.The expression of E-cadherin protein was analyzed by using immunohistochemistry.The mRNA transcription levels of E-cadherin gene in different month fetal head skin was tested by using qRT-PCR method.Results showed that the fetal head skin begins to form primary hair buds in embryonic age of 60-70 days,and form the hair bulbs structure in the embryonic age of 130 days.Secondary hair follicles differentiate out from primary hair follicles in gestational age of 80-90days;The parts of the fetal skin melanin gathering situations in different periods were observed,the hair follicles differentiation was started from head,of which lower,eyebrows,eyelashes,and horn site were the most obvious;E-cadherin protein was located on the epidermis,dermis and hair follicles,and showed high expression in epidermis,and moderate positive expression in hair follicles.E-cadherin gene mRNA transcription were uptrend in differ-ent embryonic age of yak skin hair follicle,but was lower at the embryonic age of 90 days,it was obviously below those at 70 days,120days and 130days(P<0.05).Yak head hair follicle is developed in the embryonic age of 60-70 days to form hair buds,and hair follicle development is likely forms from head.E-cadherin gene is most likely involved in the development of yak hair follicles.

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