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当前位置: 首页 > 文章 > 茶树芽叶紫化的生理生化分析及其关键酶基因的表达 生物技术通报 2015,31 (1) 86-91
Position: Home > Articles > Physiological and Biochemical Analysis of Young Shoot Purple-related and Gene Expression of Key Enzymes in Tea Plant(Camellia sinensis) Biotechnology Bulletin 2015,31 (1) 86-91

茶树芽叶紫化的生理生化分析及其关键酶基因的表达

作  者：
周琼琼;孙威江
单  位：
福建农林大学安溪茶学院;福建农林大学园艺学院
关键词：
茶树;紫色叶片;理化分析;基因表达
摘  要：
从生理生化和分子水平方面比较了茶树紫化芽叶与成熟绿色叶片的差异。结果表明,紫色幼嫩新梢中茶多酚、儿茶素总量、咖啡碱含量高于成熟绿色叶片,差异达到极显著。光合色素中,成熟绿叶样品中叶绿素及叶绿素a、b的含量、类胡萝卜素的含量极显著高于幼嫩紫叶样品,花青素含量极显著低于幼嫩紫叶;在研究的9个花青素合成途径关键酶基因中,实时荧光定量PCR分析表明,PAL、C4H、CHS、CHI、F3H、F3'H、F3'5'H、DFR和ANS在幼嫩紫叶中均呈上调表达,从而促进花青素的合成,使芽叶呈现紫色。
译  名：
Physiological and Biochemical Analysis of Young Shoot Purple-related and Gene Expression of Key Enzymes in Tea Plant(Camellia sinensis)
作  者：
Zhou Qiongqiong;Sun Weijiang;College of Horticulture,Fujian Agriculture and Forestry University;Anxi Tea College,Fujian Agriculture and Forestry University;
关键词：
tea plant;;purple leaves;;physical and chemical analysis;;gene expression
摘  要：
The young purple shoots of tea plant(Camellia sinensis)were compared to mature green leaves based on the biochemical and molecular level. Results showed that the content of tea polyphenols,catechins,caffeine and anthocyanins in the tender purple leaves were significantly higher than those in mature green leaves,while the content of chlorophyll and chlorophyll a,b,carotenoids were significantly lower than those mature green leaves. Q-PCR analysis indicated that the key genes of PAL、C4H、CHS、CHI、F3H、F3'H、F3'5'H、DFR and ANS in the anthocyanin biosynthesis were up-regulated in the tender purple leaves,which may contribute to the synthesis of anthocyanins and the color of the young shoots.

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