当前位置: 首页 > 文章 > 溶藻细菌F8与藻毒素降解菌T1的原生质体融合研究 湖北农业科学 2017,56 (6) 1033-1036
Position: Home > Articles > The Study of Protoplast Fusion with Algae-lysing Bacteria F8 and Microcystin Degrading Bacteria T1 Hubei Agricultural Sciences 2017,56 (6) 1033-1036

溶藻细菌F8与藻毒素降解菌T1的原生质体融合研究

作  者:
张安妮;沈红池;陈雪珍;周新程;张文艺
单  位:
常州大学环境与安全工程学院
关键词:
溶藻细菌;藻毒素降解菌;原生质体融合
摘  要:
为制备出兼具溶藻及降解藻毒素(MC-LR)的双效工程菌,研究不同的亲本原生质体灭活方式、不同PEG浓度、pH以及作用的温度对原生质体融合的影响,探讨溶藻细菌F8与藻毒素降解菌T1菌株的原生质体融合的适宜条件。结果表明,对F8菌株的原生质体采用45℃热灭活、T1菌株的原生质体紫外灭活,采用pH为8.0的30%的PEG溶液,融合温度为30℃,原生质体融合率可达28.17%。
译  名:
The Study of Protoplast Fusion with Algae-lysing Bacteria F8 and Microcystin Degrading Bacteria T1
作  者:
ZHANG An-ni;SHEN Hong-chi;CHEN Xue-zhen;ZHOU Xin-cheng;ZHANG Wen-yi;School of Environmental and Safety Engineering,Changzhou University;
关键词:
algae-lysing bacteria;;microcystin degrading bacteria;;protoplast fusion
摘  要:
In order to prepare double engineering bacterium which could both dissolve algae and degradation of microcystin degrading bacteria(MC-LR), the influence of different parent protoplast inactivating methods, concentrations of PEG, pH and temperature on the protoplast fusion were studied. And the suitableconditions of protoplast fusion between dissolve algae bacteria F8 and algae toxin degradation bacterium T1 strain were explored. The results indicated that when the F8 protoplast was inactivated heat by 45 ℃,T1 protoplast was under UV inactivated, pH of PEG solution was 8.0, the mass fraction of PEG solutionwas 30% and the fusion temperature was 30 ℃, the protoplast fusion rate could reach 28.17%.

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