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红树莓工厂化繁育技术体系研究

作  者:
尤淑丽;王海新;苏君伟;安玉明;赵艳
单  位:
辽宁省风沙地改良利用研究所
关键词:
红树莓;工厂化繁育;培养基
摘  要:
对红树莓的4个优良栽培品种"托拉米"、"维拉米""、"菲尔杜德""、"海尔特兹"组织培养过程中的几个主要环节进行了系统研究。结果表明:红树莓工厂化生产中,外植体采用4~6月取茎尖或9月取茎段比较适宜。茎尖分化培养采用培养基:1/2MS+BA0.75mg/L+GA30.5mg/L;茎段分化培养采用培养基:1/2MS+BA0.5mg/L+IAA0.2mg/L+GA30.5mg/L;通过正交试验得出:"维拉米"和"菲尔杜德"的最佳增殖培养基为:MS+BA0.5mg/L+IAA0.2mg/L+GA30.3mg/L;"托拉米"和"海尔特兹"的最佳增殖培养基为:MS+BA0.35mg/L+IAA0.1mg/L+GA30.3mg/L;增殖系数达到4~6;4个品种最佳生根培养基均为:1/2MS+IAA0.3mg/L+IBA0.2mg/L;平均生根率85.3%;依照试验确定蛭石是最佳移栽驯化基质,移栽成活率达到90.2%。
译  名:
Study on Technological System of Industrial Production of Red Raspberry Idaeus
作  者:
YOU Shu-li,WANG Hai-xin,SU Jun-wei,AN Yu-ming,ZHAO Yan (Liaoning Institute of Aeolian Land Improvement and Utilazation,Fuxin,Liaoning 123000)
关键词:
red raspberry;industrial production;medium
摘  要:
Systematic Study on major stage of tissue culture of excellent 4red raspberry varieties:Tullameen,Wilamette,Fertodi and Heritage.The results indicated that in industrial production,the stem tip of April to June and stem segment of September were selected as the optimum explants.and the appropriate media for respective culture stages were:for regeneration of stem tips:1/2MS+BA 0.75mg/L+GA30.5mg/L;for regeneration of stem segments:1/2MS+BA 0.5 mg/L+IAA 0.2mg/L+GA30.5mg/L;By orthogonal design concluded,for differentiation and multiplication of Tullameen and Heritage:MS+BA 0.35mg/L+IAA 0.1mg/L+GA30.3mg/L,of Wilamette and Fertodi:MS+BA 0.35 mg/L+IAA 0.1mg/L+GA30.3mg/L;the multiple for multiplication was up to 4~6;for rooting:1/2MS+IAA 0.3 mg/L+IBA 0.2mg/L;the mean rate of rooting was 85.3%;with the experiment,vermiculite was the excellent matrix,Transplanting surviving rate could reach 90.2%.

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