中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 白头翁汤及其主要成分对内皮细胞分泌细胞因子TNF-α,TXB_2和6-keto-PGF_(1α)的影响 吉林农业大学学报 2014 (3) 342-346
Position: Home > Articles > Effects of PD and Its Ingredients on LPS-induced Endothelial Cell to Secrete TNF-α,TXB_2 and 6-keto-PGF_(1α) Journal of Jilin Agricultural University 2014 (3) 342-346

白头翁汤及其主要成分对内皮细胞分泌细胞因子TNF-α,TXB_2和6-keto-PGF_(1α)的影响

作  者：
胡屹屹;穆祥;胡元亮
单  位：
南京农业大学动物医学院;江苏省农业科学院兽医研究所·农业部兽用生物制品工程技术重点实验室·国家兽用生物制品工程技术研究中心;北京农学院兽医学中医药北京市重点实验室
关键词：
白头翁汤;内毒素;肿瘤坏死因子-α;血栓素B2;6-keto-PGF1α
摘  要：
为探讨白头翁汤及其主要成分对细菌内毒素(LPS)导致的机体炎症等病理损伤的治疗作用。培养内皮细胞至单层融合状态,加入内毒素刺激,3 h后加入高、中、低3种浓度的白头翁汤、白头翁皂苷B4、白头翁素、小檗碱、药根碱、巴马汀、秦皮甲素和秦皮乙素,继续培养21 h后收集细胞上清液,用ELISA方法测定TNF-α,TXB2和6-keto-PGF1α的含量。结果表明:白头翁汤高剂量组、白头翁皂苷B4高剂量组和小檗碱高剂量组的TNF-α含量极显著低于LPS对照组(P<0.01);白头翁汤中剂量组、白头翁皂苷B4中剂量组、小檗碱中剂量组、药根碱中剂量组和秦皮甲素高剂量组显著低于LPS对照组(P<0.05);白头翁汤高剂量组和巴马汀高剂量组的TXB2含量极显著低于LPS对照组(P<0.01);白头翁汤中剂量组、白头翁素高剂量组、小檗碱中剂量组、药根碱高剂量组和中剂量组、巴马汀中剂量组显著低于LPS对照组(P<0.05);秦皮乙素高剂量组的6-keto-PGF1α含量显著低于LPS对照组(P<0.05)。说明白头翁汤及其主要成分对LPS体外诱导内皮细胞产生炎性细胞因子具有明显的抑制作用,可能对TNF-α,TXB2和6-keto-PGF1α介导的机体炎症、凝血和休克等病理损伤具有治疗作用。
译  名：
Effects of PD and Its Ingredients on LPS-induced Endothelial Cell to Secrete TNF-α,TXB_2 and 6-keto-PGF_(1α)
作  者：
HU Yi-yi;MU Xiang;HU Yuan-liang;Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Veterinary Biological Engineering and Technology,Ministry of Agriculture,National Center for Engineering Research of Veterinary Bio-products;Beijing Key Laboratory of Traditional Chinese Veterinary Medicine,Beijing Agricultural University;College of Veterinary Medicine,Nanjing Agricultural University;
关键词：
pulsatillae decoction;;LPS;;TNF-α;;TXB2;;6-keto-PGF1α
摘  要：
The aim of the research was to investigate the anti-inflammatory effects of Pulsatillae Decoction(PD).The levels of TNF-α,TXB2 and 6-keto-PGF1αsecreted by cultured rat intestinal microvascular endothelial cells were determined after treatment with its active ingredients,namely anemoside B4,anemonin,berberine,jatrorrhizine,palmatine,aesculin,and esculetin.Endothelial cell was challenged with 1 μg /mL LPS for 3 h,then treated respectively with PD at three concentrations(1,5 and 10 mg /mL) and its seven ingredients at three concentrations(1,5 and 10 μg /mL).The cells were incubated at 37 ℃ in a cell incubator for 21 h.The supernatants were collected and the contents of TNF-α,TXB2 and 6-keto-PGF1αwere analyzed by ELISA kits.The results showed that compared with LPS control group,the TNF-α contents significantly decreased in the middle-and high-dose groups of PD,the middle-and high-dose groups of Anemoside B4,the middleand high-dose groups of Berberine,the middle-dose group of Jateorhizine and the high-dose group of Aesculin;the TXB2 contents significantly decreased in the middle-and high-dose groups of PD,the high-dose group of Anemonin,the middle-dose group of Berberine,the middle-and high-dose groups of Jateorhizine and the middle-and high-dose groups of Palmatine;The 6-keto-PGF1αcontents significantly decreased in the high-dose group of Esculetin.The research suggested that PD may effectively reduce inflammation,coagulation and shock response via these cytokines.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46