Analgesic roles of aspirin eugenol ester and its mechanisms

LI Jian-yong1,YU Yuan-guang1,3,WANG Qi-wen2,YANG Ya-jun1,WEI Xiao-juan1,ZHOU Xu-zheng1,NIU Jian-rong1,LI Bing1,ZHANG Ji-yu1,YE De-he3 (1.Key Laboratory of New Animal Drug Project,Lanzhou Institute of Animal Science and Veterinary Pharmaceutics,Chinese Academy of Agricultural Sciences,Lanzhou 730050,China;2.Institute of Grassland Ecological Research,Bijie University,Bijie 551700,China;3.College of Animal Medicine,Agricultural University of Gansu,Lanzhou 730070,China)

aspirin eugenol ester;analgesia;mechanism

The analgesic roles of aspirin eugenol ester(AEE) were investigated by using Writhing test and Hot plate test,while the influence of some drugs,including Diethyldithiocarbamate(DDC),haloperidol(Hal),L-tryptophan(L-Trp) and cyproheptadine(Cyp),on the analgesic effects under heat stimulation were examined.Also,the contents of PGE2 in the whole brain of mice were measured by ultraviolet spectrophotometry.The result showed that both the writhing induced by acetic acid and the paining by thermal stimulation in mice were significantly eased by AEE;the contents of PGE2 in the whole brain of mice were decreased;the algesa induced by DDC was inhibited;a combined effect between Hal and AEE existed,while L-Trp/CYP had significant antagonism to AEE.It suggested that AEE has an obvious analgesic effect which may be related to decreasing the production of the PGE2 in the whole brain of rats,raising the contents of norepinephrine(NE) and blocking-up the dopamine(DA) receptor.