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当前位置: 首页 > 文章 > 实时荧光聚合酶链式反应法检测食品中猪源性成分 食品科学 2013,34 (8) 224-227
Position: Home > Articles > Detection for Pig-Derived Components in Foods by Real-Time Polymerase Chain Reaction FOOD SCIENCE 2013,34 (8) 224-227

实时荧光聚合酶链式反应法检测食品中猪源性成分

作  者：
范丽丽;李培;傅春玲;丁洪流;陈英
单  位：
苏州市产品质量监督检验所;苏州大学医学部公共卫生学院
关键词：
实时荧光聚合酶链式反应;猪;线粒体细胞色素b基因;食品掺假
摘  要：
针对猪线粒体细胞色素b基因序列设计特异的引物和探针,建立食品中猪源性成分实时荧光聚合酶链式反应检测方法,并经特异性和敏感性试验验证其可行性。结果表明:该体系可扩增猪肉DNA片段,长度为98bp,其他常见畜、禽肉成分均无法正常扩增。该体系灵敏度低至1pg,且阴性样品扩增后的Ct值限制在35循环以后。对于各模拟肉类样品中掺杂的猪源性成分,其检测限均达1%,经市售加工食品检测验证,表明所建立的猪引物探针体系具有特异性好、灵敏度高、快速、高效等优点,可用于对食品中猪源性成分的掺假鉴别检测。
译  名：
Detection for Pig-Derived Components in Foods by Real-Time Polymerase Chain Reaction
作  者：
FAN Li-li1,LI Pei2,FU Chun-ling1,,DING Hong-liu2,CHEN Ying2(1.School of Public Health,Medical College of Soochow University,Suzhou 215123,China;2.Suzhou Products Quality Supervision and Inspection Institute,Suzhou 215128,China)
关键词：
real-time polymerase chain reaction;pig;mitochondrial cytochrome b gene;food adulteration
摘  要：
Species-specific real-time PCR(TaqMan) assay was developed for the detection of pig-derived components in foods.Primers and Taqman probe of this assay were designed with pig conservative regions of the mitochondrial cytochrome b(Cytb) gene(amplicon = 98 base pairs).The specificity was evaluated and achieved without amplification on DNA from other meats.Results showed that this method revealed a high sensitivity and could detect 1 pg of pork template DNA,while Ct(cycle threshold) values of negative samples were limited to 35 cycles.Applying this assay method,the recovery rate of DNA extracted from meat mixtures was up to 1% pork spiked in other species.Therefore,it is a potentially reliable and suitable technique in routine food analysis for the detection of pork in foods.

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