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当前位置: 首页 > 文章 > 基于生物量的白灵菇菌株筛选及其深层发酵培养条件优化 食品科学 2013,34 (23) 221-227
Position: Home > Articles > Strain Screening of Pleurotus nebrodensis and Optimization of Submerged Culture Conditions for Biomass Prodution FOOD SCIENCE 2013,34 (23) 221-227

基于生物量的白灵菇菌株筛选及其深层发酵培养条件优化

作  者：
冀宏;赵黎明
单  位：
天津大学管理与经济学部;常熟理工学院生物与食品工程学院
关键词：
白灵菇;深层发酵;生物量;正交试验;响应面法
摘  要：
以深层发酵菌丝体生物量为考察指标,对白灵菇液体深层发酵的培养基及发酵条件进行优化。通过正交试验(L9(34))确定发酵培养基最佳配方。在此基础上,基于三因素三水平的Box-Behnken设计试验,采用响应曲面分析法对影响白灵菇菌丝体生长的发酵温度、摇瓶转速和发酵周期进行优化。结果表明:最佳培养基组分为(g/100mL):可溶性淀粉3.50、牛肉膏0.40、磷酸二氢钾0.25、硫酸镁0.25;当摇瓶发酵温度为26℃、转速为160r/min,发酵周期7.5d(180h)时,发酵菌丝体生物量可达1.6004g/100mL。
译  名：
Strain Screening of Pleurotus nebrodensis and Optimization of Submerged Culture Conditions for Biomass Prodution
作  者：
JI Hong;ZHAO Li-ming;Department of Management and Economics,Tianjin University;School of Biology and Food Engineering,Changshu Institute of Technology;
关键词：
Pleurotus nebrodensis;;submerged fermentation;;mycelia biomass;;orthogonal array design;;response surface methodology
摘  要：
Pleurotus nebrodensis Tianshan No.2 from Wuan, Hebei province was selected as the fastest-growing of 19 strains. The culture medium for submerged fermentation of strain Tianshan No.2 were optimized for the maximum biomass(dry cell weight, DCW) production using an orthogonal array design L 9(3 4), and th ree culture conditions including temperature, shaking speed and time were optimized by response surface analysis based on a three-variable, three-level Box-Behnken experimental design. The results showed that the optimal medium composition(per 100 mL) was 3.50 g of soluble starch, 0.40 g of beef extract, 0.25 g of monopotassium phosphate and 0.25 g of magnesium sulfate. The mycelia biomass of strain Tianshan No.2 was 1.6004 g/100 mL after 7.5 d(180 h) of shaking culture at 26 ℃ with a shaking speed of 160 r/min using the optimized medium.

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