作 者：
缑绪卓;严子成;况玉;曹锐;陈巧灵;杨雅丽;周嘉裕;廖海
关键词：
Kunitz蛋白酶抑制剂;大豆;蒺藜苜蓿;基因组;生物信息学
摘 要：
Kunitz蛋白酶抑制剂(Kunitz protease inhibitor,KPI)基因是植物中具有抗逆功能的基因家族之一.为研究KPI基因家族的进化与功能,对大豆和蒺藜苜蓿的KPI基因家族成员进行比较基因组学分析.利用生物信息学方法鉴定大豆与蒺藜苜蓿的KPI基因家族成员,并对其染色体定位进行分析,确定了大豆与蒺藜苜蓿KPI基因成员的直系同源与旁系同源基因及保守基序,并对大豆与蒺藜苜蓿不同组织及不同胁迫条件下KPI基因家族成员的表达情况进行了分析.结果显示,大豆、蒺藜苜蓿各含有48、46个KPI基因成员,它们在染色体上分布不均匀,并存在串联重复而形成的基因簇;大豆与蒺藜苜蓿有11对直系同源基因,二者各有14对旁系同源基因;大豆和蒺藜苜蓿均含有3个结构基序,靠近N末端的2个结构基序为大豆与蒺藜苜蓿共有,而靠近C末端的第3个结构基序位置有较大差异,可以作为区分大豆与蒺藜苜蓿KPI成员的一段特征性标记;EST(Express sequence tags,表达序列标签)分析结果表明,KPI基因家族成员主要在根、种子中表达;干旱与盐胁迫条件下,KPI成员的数量增加,推测KPI参与了大豆与蒺藜苜蓿对干旱与盐胁迫的逆境响应过程.
作 者：
GOU Xuzhuo;YAN Zicheng;KUANG Yu;CAO Rui;CHEN Qiaoling;YANG Yali;ZHOU Jiayu;LIAO Hai;School of Life Sciences and Engineering,Southwest Jiaotong University;
单 位：
GOU Xuzhuo%YAN Zicheng%KUANG Yu%CAO Rui%CHEN Qiaoling%YANG Yali%ZHOU Jiayu%LIAO Hai%School of Life Sciences and Engineering,Southwest Jiaotong University
关键词：
Kunitz protease inhibitor;;Glycine max;;Medicago truncatula;;Genome;;Bioinformatics
摘 要：
Kunitz protease inhibitor(KPI) was one of gene families involved in anti-stress function in plants.In the article,comparative genomic analysis on the KPI genes from Glycine max and Medicago truncatula was carried out based on the whole genomes of G.max and M.truncatula.Firstly,according to the bioinformatics methods,the KPI genes in G.max and M.truncatula were identified and their chromosome location was analyzed,respectively.Then,the orthologous and paralogous genes,and conserved motifs were determined.At last,the expressional level of each member in different organs and under various stresses was analyzed based on the ESTs(Express sequence tags) data.According to the results,a total of 48 and 46 KPI genes were identified in G.max and M.truncatula,respectively.These genes were unevenly distributed on the chromosome,and some formed gene clusters by tandem repeats.There were 11 pairs of orthologous genes between G.max and M.truncatula,and 14 paralogous genes in both G.max and M.truncatula.Three motifs were discovered in KPIs,in which two motifs adjacent to the N-terminus were highly conserved,while the third motif near the C-terminus showed location diversity and might be applied as a characteristic marker to distinguish the KPIs from G.max and M.truncatula.The results of EST analysis indicated that most of the KPIs expressed in roots and seeds.After drought and salt stress,the increased number of KPIs indicated that the KPIs might be involved in the stress response to drought and salt stress in G.max and M.truncatula.
