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当前位置: 首页 > 文章 > 水稻条斑病菌异柠檬酸脱氢酶在致病性中的功能分析 植物病理学报 2016,46 (5) 624-633
Position: Home > Articles > Functional analysis of isocitrate dehydrogenase from rice pathogen Xanthomonas oryzae pv. oryzicola in pathogenicity Acta Phytopathologica Sinica 2016,46 (5) 624-633

水稻条斑病菌异柠檬酸脱氢酶在致病性中的功能分析

作  者：
郭威;邹丽芳;陈功友
单  位：
南京农业大学植物保护学院/农业部病虫监测与治理重点开放实验室上海交通大学农业与生物学院;上海交通大学农业与生物学院;上海交通大学医学院附属第九人民医院
关键词：
水稻条斑病菌;异柠檬酸脱氢酶;致病性;碳水化合物;转录水平
摘  要：
异柠檬酸脱氢酶(isocitrate dehydrogenase,IcdH)催化异柠檬酸转化成α-酮戊二酸,参与碳代谢途径末端的三羧酸(tricarboxylic acid,TCA)循环。然而,编码异柠檬酸脱氢酶基因是否参与水稻条斑病菌(Xanthomonas oryzae pv.oryzicola,Xoc)的致病性,我们并不清楚。为了阐明IcdH的作用,通过同源重组技术获得了Xoc的icdH基因缺失突变体(RΔicdH),并对该突变体进行了相关功能研究。研究表明:该突变体不能利用苹果酸、丙酮酸和柠檬酸,在寄主水稻上的生长能力和致病力相对于野生型均显著降低,其游动性也显著减弱;功能互补子恢复RΔicdH的上述表型至野生型水平;Real-time PCR结果显示,六碳单糖、蔗糖、苹果酸、丙酮酸与柠檬酸能显著诱导icdH基因的转录表达;与水稻细胞互作时icdH基因受诱导表达,并受HrpX和HrpG负调控。这些结果说明:icdH基因是Xoc获取碳源和在寄主水稻上具有致病性所需的。
译  名：
Functional analysis of isocitrate dehydrogenase from rice pathogen Xanthomonas oryzae pv. oryzicola in pathogenicity
作  者：
GUO Wei;ZOU Li-fang;CHEN Gong-you;College of Chemistry and Life Sciences,Zhejiang Normal University;College of Agriculture and Biology,Shanghai Jiao Tong University;
关键词：
Xanthomonas oryzae pv.oryzicola;;isocitrate dehydrogenase(IcdH);;pathogenicity;;carbohydrate;;transcript level
摘  要：
Isocitrate dehydrogenase(IcdH) converts isocitrate to α-oxoglutarate,which is implicated in the tricarboxylic acid(TCA) cycle of terminal carbon metabolic pathways.However,it is unclear whether the IcdH-coding gene is involved in pathogenicity of Xanthomonas oryzae pv.oryzicola(Xoc).To clarify the roles of IcdH,we generated the icdH deletion mutant(R△icdH) via allelic exchange and performed functional analysis with the mutant.Our findings indicated that the resulting icdH mutant not only failed to use pyruvate,malate or citrate,but also displayed an impaired bacterial growth,an attenuated virulence in host rice,and a reduced bacterial motility compared with the wild-type.The lost properties mentioned above in R△icdH were completely restored to the wild-type level by the presence of icdH in trans.Real-time PCR assays confirmed that transcript level of icdH was significantly induced by C_6-sugar,sucrose,malate,pyruvate or citrate.The expression of icdH was also inducible in rice suspension cells rather than in a nutrient-rich(NB) medium and negatively regulated by HrpG and HrpX.Our results suggest that icdH is required for Xoc to obtain carbohydrate nutrient,and to cause pathogenicity in host rice.

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